Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines
Previous studies have shown that expression of RPL37A gene was downregulated in nasopharyngeal carcinoma cell lines and it had been reported that genetic mutation of coding sequences in ribosomal protein gene RPSJ9 is the cause of dysregulation of gene expression in Diamond-Blackfan Anemia. The o...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8211/11/Kiung%20Siong%20Siang%20%28full%29.pdf http://ir.unimas.my/id/eprint/8211/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Previous studies have shown that expression of RPL37A gene was downregulated in
nasopharyngeal carcinoma cell lines and it had been reported that genetic mutation of
coding sequences in ribosomal protein gene RPSJ9 is the cause of dysregulation of gene
expression in Diamond-Blackfan Anemia. The objective of this study is to determine
whether mutation in coding sequences of RPL37A gene is the cause of its differential
expression in nasopharyngeal carcinoma by isolating, sequencing and analyzing the coding
sequences of the gene from normal nasopharyngeal epithelial cell lines and nasopharyngeal
carcinoma cell lines. Results from this study show that there is no difference in the
nucleotide sequence in the coding region of RPL37A gene from both types of
nasopharyngeal cell lines and therefore indicates that mutation in the coding region of the
RPL37A gene is not the cause of its differential expression in nasopharyngeal carcinoma. |
|---|