Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia.
A better description of the extent and structure of genetic diversity in dengue virus (DENV) in endemic settings is central to its eventual control. To this end we determined the complete coding region sequence of 187 DENV-2 genomes and 68 E genes from viruses sampled from Vietnamese patients betwee...
Saved in:
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | E-Article |
Language: | English |
Published: |
PLOS
2010
|
Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/7008/1/Emergence%20of%20the%20Asian%201%20Genotype%20of%20Dengue%20Virus%20Serotype%202%20in%20Viet%20Nam_%20In%20Vivo%20Fitness%20Advantage%20and%20Lineage%20Replacement%20in%20South-East%20Asia.pdf http://ir.unimas.my/id/eprint/7008/ http://www.ncbi.nlm.nih.gov/pubmed/20651932 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.unimas.ir.7008 |
---|---|
record_format |
eprints |
spelling |
my.unimas.ir.70082015-04-14T07:56:24Z http://ir.unimas.my/id/eprint/7008/ Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. Hang, Vu Thi Ty Holmes, Edward C. Veasna, Duong Quy, Nguyen Thien Hien, Tran Tinh Quail, Michael Churcher, Carol Parkhill, Julian Cardosa, Mary Jane Farrar, Jeremy Wills, Bridget Lennon, Niall J. Birren, Bruce W. Buchy, Philippe Henn, Matthew R. Simmons, Cameron P. Q Science (General) QR355 Virology R Medicine (General) A better description of the extent and structure of genetic diversity in dengue virus (DENV) in endemic settings is central to its eventual control. To this end we determined the complete coding region sequence of 187 DENV-2 genomes and 68 E genes from viruses sampled from Vietnamese patients between 1995 and 2009. Strikingly, an episode of genotype replacement was observed, with Asian 1 lineage viruses entirely displacing the previously dominant Asian/American lineage viruses. This genotype replacement event also seems to have occurred within DENV-2 in Thailand and Cambodia, suggestive of a major difference in viral fitness. To determine the cause of this major evolutionary event we compared both the infectivity of the Asian 1 and Asian/American genotypes in mosquitoes and their viraemia levels in humans. Although there was little difference in infectivity in mosquitoes, we observed significantly higher plasma viraemia levels in paediatric patients infected with Asian 1 lineage viruses relative to Asian/American viruses, a phenotype that is predicted to result in a higher probability of human-to-mosquito transmission. These results provide a mechanistic basis to a marked change in the genetic structure of DENV-2 and more broadly underscore that an understanding of DENV evolutionary dynamics can inform the development of vaccines and anti-viral drugs. PLOS 2010-07 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/7008/1/Emergence%20of%20the%20Asian%201%20Genotype%20of%20Dengue%20Virus%20Serotype%202%20in%20Viet%20Nam_%20In%20Vivo%20Fitness%20Advantage%20and%20Lineage%20Replacement%20in%20South-East%20Asia.pdf Hang, Vu Thi Ty and Holmes, Edward C. and Veasna, Duong and Quy, Nguyen Thien and Hien, Tran Tinh and Quail, Michael and Churcher, Carol and Parkhill, Julian and Cardosa, Mary Jane and Farrar, Jeremy and Wills, Bridget and Lennon, Niall J. and Birren, Bruce W. and Buchy, Philippe and Henn, Matthew R. and Simmons, Cameron P. (2010) Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. Neglected Tropical Diseases, 4 (7). ISSN 1935-2735 http://www.ncbi.nlm.nih.gov/pubmed/20651932 10.1371/journal.pntd.0000757 |
institution |
Universiti Malaysia Sarawak |
building |
Centre for Academic Information Services (CAIS) |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sarawak |
content_source |
UNIMAS Institutional Repository |
url_provider |
http://ir.unimas.my/ |
language |
English |
topic |
Q Science (General) QR355 Virology R Medicine (General) |
spellingShingle |
Q Science (General) QR355 Virology R Medicine (General) Hang, Vu Thi Ty Holmes, Edward C. Veasna, Duong Quy, Nguyen Thien Hien, Tran Tinh Quail, Michael Churcher, Carol Parkhill, Julian Cardosa, Mary Jane Farrar, Jeremy Wills, Bridget Lennon, Niall J. Birren, Bruce W. Buchy, Philippe Henn, Matthew R. Simmons, Cameron P. Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. |
description |
A better description of the extent and structure of genetic diversity in dengue virus (DENV) in endemic settings is central to its eventual control. To this end we determined the complete coding region sequence of 187 DENV-2 genomes and 68 E genes from viruses sampled from Vietnamese patients between 1995 and 2009. Strikingly, an episode of genotype replacement was observed, with Asian 1 lineage viruses entirely displacing the previously dominant Asian/American lineage viruses. This genotype replacement event also seems to have occurred within DENV-2 in Thailand and Cambodia, suggestive of a major difference in viral fitness. To determine the cause of this major evolutionary event we compared both the infectivity of the Asian 1 and Asian/American genotypes in mosquitoes and their viraemia levels in humans. Although there was little difference in infectivity in mosquitoes, we observed significantly higher plasma viraemia levels in paediatric patients infected with Asian 1 lineage viruses relative to Asian/American viruses, a phenotype that is predicted to result in a higher probability of human-to-mosquito transmission. These results provide a mechanistic basis to a marked change in the genetic structure of DENV-2 and more broadly underscore that an understanding of DENV evolutionary dynamics can inform the development of vaccines and anti-viral drugs. |
format |
E-Article |
author |
Hang, Vu Thi Ty Holmes, Edward C. Veasna, Duong Quy, Nguyen Thien Hien, Tran Tinh Quail, Michael Churcher, Carol Parkhill, Julian Cardosa, Mary Jane Farrar, Jeremy Wills, Bridget Lennon, Niall J. Birren, Bruce W. Buchy, Philippe Henn, Matthew R. Simmons, Cameron P. |
author_facet |
Hang, Vu Thi Ty Holmes, Edward C. Veasna, Duong Quy, Nguyen Thien Hien, Tran Tinh Quail, Michael Churcher, Carol Parkhill, Julian Cardosa, Mary Jane Farrar, Jeremy Wills, Bridget Lennon, Niall J. Birren, Bruce W. Buchy, Philippe Henn, Matthew R. Simmons, Cameron P. |
author_sort |
Hang, Vu Thi Ty |
title |
Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. |
title_short |
Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. |
title_full |
Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. |
title_fullStr |
Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. |
title_full_unstemmed |
Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in South-East Asia. |
title_sort |
emergence of the asian 1 genotype of dengue virus serotype 2 in viet nam: in vivo fitness advantage and lineage replacement in south-east asia. |
publisher |
PLOS |
publishDate |
2010 |
url |
http://ir.unimas.my/id/eprint/7008/1/Emergence%20of%20the%20Asian%201%20Genotype%20of%20Dengue%20Virus%20Serotype%202%20in%20Viet%20Nam_%20In%20Vivo%20Fitness%20Advantage%20and%20Lineage%20Replacement%20in%20South-East%20Asia.pdf http://ir.unimas.my/id/eprint/7008/ http://www.ncbi.nlm.nih.gov/pubmed/20651932 |
_version_ |
1644510189349502976 |
score |
13.18916 |