Production of amylase by bacillus sp. Under solid-state fermentation (SSF) of sago waste

• Main Author: Lau, Nge Kai
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, UNIMAS 2012
• Subjects: Q Science (General); QH301 Biology; TD Environmental technology. Sanitary engineering
• Online Access: http://ir.unimas.my/id/eprint/6245/1/Lau%20Nge%20ft.pdf; http://ir.unimas.my/id/eprint/6245/

Amylase is one of the most commercially valuable hydrolase enzymes which have been applied in various industries especially within starch processing industry. However, there are problems associated with the production of amylase such as high production costs. The study is carried out to investigate optimal amylase production by amylolytic Bacillus sp. under Solid-State Fermention (SSF) using cheap and abundance sago waste as substrates. Set of parameters including optimum incubation periods, optimum temperature, optimum pH, and optimum inoculums concentration are purposed to determine the optimal condition for the amylase production by the co-culture of amylolytic Bacillus sp. through enzymes activities test or enzymes assay. Results showed that Bacillus amyloliquefaciens UMAS 1002 and Bacillus licheniformis UMAS P7 are best amylase producing strains and the optimal condition for α-amylase production by co-culture of these Bacillus strains through SSF using sago waste are 37oC, acetate buffer with pH 7, 10% (v/v) of inoculums sizes and 3 days of incubation with 3.015 IU/ml of α-amylase production.