Production of amylase by bacillus sp. Under solid-state fermentation (SSF) of sago waste
Amylase is one of the most commercially valuable hydrolase enzymes which have been applied in various industries especially within starch processing industry. However, there are problems associated with the production of amylase such as high production costs. The study is carried out to investiga...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6245/1/Lau%20Nge%20ft.pdf http://ir.unimas.my/id/eprint/6245/ |
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| Summary: | Amylase is one of the most commercially valuable hydrolase enzymes which
have been applied in various industries especially within starch processing industry.
However, there are problems associated with the production of amylase such as high
production costs. The study is carried out to investigate optimal amylase production by
amylolytic Bacillus sp. under Solid-State Fermention (SSF) using cheap and abundance
sago waste as substrates. Set of parameters including optimum incubation periods,
optimum temperature, optimum pH, and optimum inoculums concentration are purposed to
determine the optimal condition for the amylase production by the co-culture of amylolytic
Bacillus sp. through enzymes activities test or enzymes assay. Results showed that Bacillus
amyloliquefaciens UMAS 1002 and Bacillus licheniformis UMAS P7 are best amylase
producing strains and the optimal condition for α-amylase production by co-culture of
these Bacillus strains through SSF using sago waste are 37oC, acetate buffer with pH 7, 10%
(v/v) of inoculums sizes and 3 days of incubation with 3.015 IU/ml of α-amylase
production. |
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