Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis
Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towar...
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Faculty of Food Science & Technology, UPM
2011
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my.unimas.ir.400142022-10-02T23:49:52Z http://ir.unimas.my/id/eprint/40014/ Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis Samuel, Lihan Marian Madonna, Mobilik Lesley, Maurice Bilung Son, Radu Kasing, Apun QR Microbiology Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towards some of the antibiotics tested, none of the isolates showed resistant towards norfloxacin, sulphametoxazole/trimethoprim and chloramphenicol. RAPD-PCR analysis using single primer and primers combination clustered the E. coli isolates into 3 and 5 groups, respectively. The results of this study suggest that the E. coli isolates from the catfish and their surrounding environment derived from a mixture of sensitive and resistant strains with diverse genetic contents. The use of the RAPD analysis is sufficiently discriminatory for the typing of the E. coli isolates. Faculty of Food Science & Technology, UPM 2011 Article PeerReviewed text en http://ir.unimas.my/id/eprint/40014/3/Characterization%20of%20-%20Copy.pdf Samuel, Lihan and Marian Madonna, Mobilik and Lesley, Maurice Bilung and Son, Radu and Kasing, Apun (2011) Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis. International Food Research Journal, 18 (3). pp. 971-976. ISSN 2231 7546 http://www.ifrj.upm.edu.my/18%20(03)%202011/(16)IFRJ-2010-089.pdf |
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QR Microbiology Samuel, Lihan Marian Madonna, Mobilik Lesley, Maurice Bilung Son, Radu Kasing, Apun Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
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Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towards some of the antibiotics tested, none of the isolates showed resistant towards norfloxacin, sulphametoxazole/trimethoprim and chloramphenicol. RAPD-PCR analysis using single primer and primers combination clustered the E. coli isolates into 3 and 5 groups, respectively. The results of this study suggest that the E. coli isolates from the catfish and their surrounding environment derived from a mixture of sensitive and resistant strains with diverse genetic contents. The use of the RAPD analysis is sufficiently discriminatory for the typing of the E. coli isolates. |
format |
Article |
author |
Samuel, Lihan Marian Madonna, Mobilik Lesley, Maurice Bilung Son, Radu Kasing, Apun |
author_facet |
Samuel, Lihan Marian Madonna, Mobilik Lesley, Maurice Bilung Son, Radu Kasing, Apun |
author_sort |
Samuel, Lihan |
title |
Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
title_short |
Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
title_full |
Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
title_fullStr |
Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
title_full_unstemmed |
Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
title_sort |
characterization of escherichia coli isolated from cultured catfish by antibiotic resistance and rapd analysis |
publisher |
Faculty of Food Science & Technology, UPM |
publishDate |
2011 |
url |
http://ir.unimas.my/id/eprint/40014/3/Characterization%20of%20-%20Copy.pdf http://ir.unimas.my/id/eprint/40014/ http://www.ifrj.upm.edu.my/18%20(03)%202011/(16)IFRJ-2010-089.pdf |
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1746213624980963328 |
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13.19449 |