Effect of Peptone Supplementation on Bioethanol Fermentation using Sago Frond Sap

• Main Author: Norfahirah, Lamdani
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
• Subjects: TP Chemical technology
• Online Access: http://ir.unimas.my/id/eprint/39871/1/NORFAHIRAH%20BINTI%20LAMDANI%2024pgs.pdf; http://ir.unimas.my/id/eprint/39871/2/NORFAHIRAH%20BINTI%20LAMDANI%20ft.pdf; http://ir.unimas.my/id/eprint/39871/

Utilization of lignocellulosic biomass is preferable to yield bioethanol since it is easily available and able to minimize environmental pollution. However, lignocellulosic biomass including sago starch, sago ‘hampas’ or sago in form of hydrolysate involving high cost of production due to need to undergo pre-treatment. Thus, to minimize the cost needed, potential of sago frond sap as a substrate are currently being studied. Related to our study, it is not only just focusing on the potential of sago frond sap for yeast growth but the impact of adding peptone towards yeast growth and bioethanol production were also being highlighted. Technically, yeast required nitrogen source such as peptone to grow as well as yielding ethanol. Batch fermentation was performed by exposing yeast, Saccharomyces cerevisiae (S. cerevisiae) with two types of fermentation media consisting of 100 mL sago frond sap only and other media uses similar amount of substrate but being supplemented with 5 g/L peptone. During fermentation, the temperature was retained at 30ºC with a pH of 5.5. Based on the result, S. cerevisiae able to grow in sago frond sap producing ethanol. In fermentation media supplemented with 5 g/L peptone, both S. cerevisiae growth (9.1 g/L) and ethanol production (11.01 g/L) was enhanced compared to control media (Dry Cell Weight, DCW - 3.9 g/L, Ethanol production - 4.36 g/L). It is due to peptone provide diverse form of nutrients essential for yeast growth as well as ethanol production. To conclude, sago frond sap can become feedstock of interest which is relevant to be used in bioethanol industry. Also, presence of peptone is required to induce higher yield of yeast biomass as well as ethanol.