Lipid Accumulation in the Oleaginous Yeast Lipomyces starkeyi MV-5 Cultured in Sago Media
Biodiesel produced from plant oil, animal fats and microorganisms is a promising alternative to replace fossil fuel. However, since biodiesel feedstock from plant and animal compete with the food production supplies, another possible alternative is to use the oleaginous microorganisms. Among the o...
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Format: | Final Year Project Report |
Language: | English |
Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2017
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/32046/2/Lipid%20Accumulation%20in%20the%20Oleaginous%20Yeast%20Lipomyces%20starkeyi%20MV-5%20Cultured%20in%20Sago%20Media%28fulltext%29.pdf http://ir.unimas.my/id/eprint/32046/ |
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Summary: | Biodiesel produced from plant oil, animal fats and microorganisms is a promising alternative to replace fossil
fuel. However, since biodiesel feedstock from plant and animal compete with the food production supplies,
another possible alternative is to use the oleaginous microorganisms. Among the oleaginous microorganisms,
oleaginous yeast Lipomyces starkeyi is of interest as it can accumulate more lipids and utilize the low-valued
nutrient source such as the industrial waste. In this study, a mutant strain of L. starkeyi which is L. starkeyi
MV-5 was used to compare the lipid production in two different carbon sources; sago effluent and sago
starch. Since the starch percentage of sago effluent used was approximately 1.8%, 1.8% sago starch was
prepared to compare their efficiency. After 7 days of propagation stage, the optimum growth of L. starkeyi
MV-5 was detected at 120 h. In the lipid accumulation stage, sago effluent and 1.8% sago starch were used as
the substrate where the highest biomass ofL. starkeyi MV-5 were recorded at 168 hat 1.20 gIL and 1.55 gIL
respectively. Whereas, the highest lipid extracted from L. starkeyi MV-5 were 0.25 giL and 0.45 gIL
respectively. Phenol-sulphuric acid test was then done to determine the starch consumption of L. starkeyi
MV-5. Based on these results, the lipid content ofL. starkeyi MV-5 cultured in sago effluent and 1.8% sago
starch were 20.83% and 29.00% respectively. Thus, L. starkeyi MV-5 cultured in 1.8% sago starch gave
higher biomass and higher lipid compared to in 1.8% sago effluent. |
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