Polymorphism in the Tumor Necrosis Factor alpha promoter region and its Influence on Colorectal Cancer Predispositiom risk in Malaysian Population

• Main Authors: Mohd Aminudin, Mustapha, Siti Nurfatimah, Mohd Shahpudin, Ahmad Aizat, Abdul Aziz, Murali Krishna, V Bhavaraju, Venkatesh, R. Naik, Zaidi, Zakaria, A. Shanwani, Mohd Sidek, Mohammad Radzi, Abu Hassan, Ravindran, Ankathil
• Format: E-Article
• Language: English
• Published: Japan International Cultural Exchange Foundation (JICEF) 2011
• Subjects: R Medicine (General)
• Online Access: http://ir.unimas.my/id/eprint/30228/1/IMJ%20December%202011.pdf; http://ir.unimas.my/id/eprint/30228/; https://seronjihou.com/home/%e5%87%ba%e7%89%88%e7%89%a9/imj-international-medical-journal/

Objective: A case control study was designed to investigate the TNF-,1 -308 G>A polymorphism allele frequencies and to determine the influence of the polymorphic gcnot.ype on sporadic CRC susceptibility risk in Malaysian population. Material. and Method!: Peripheral blood samples of 164 normal controls and 161 clinically and histopathologically con­firmed CRC patients were genotyped for TNF-u -308 G>A polymorphism employing allele specific PCR. The relative associa­tions of various genotypes with CRC susceptibility risk was determined by calculating Odds Ratios. Corresponding chi-square tests on the CRC patients and controls were carried out and 95% confidence interval (95% CI) were determined using Fisher e,acts tests. Results: On comparing the frequencies of genotypes of patients and controls, the homozygous ,·ariant AA was significantly higher in CRC patients (p = 0.030) compared to controls. On investigating the association of the polymorphic genotypes with CRC susceptibility risk, the homozygous variant TNF-a -308 AA showed significantly increased risk with OR 2.5842. Conclusion: Our results suggest that, pol) morphic genotJpe of inflammation response gene TNF-a is significantly associat­ed with CRC susceptibility risk and could be considered as a high risk variant for CRC predisposition.