Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5
Japanese encephalitis virus (JEV) causes central nervous system neuronal injury and inflammation. A clear understanding of neuronal responses to JEV infection remains elusive. Using the Affymetrix array to investigate the transcriptome of infected SK-N-MC cells, 1316 and 2737 dysregulated genes (≥...
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Online Access: | http://ir.unimas.my/id/eprint/22847/1/Shu%20Pin%20Yu.pdf http://ir.unimas.my/id/eprint/22847/ https://www.journals.elsevier.com/virology |
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my.unimas.ir.228472021-05-25T12:18:38Z http://ir.unimas.my/id/eprint/22847/ Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 Shu, Pin Yu Kien, Chai Ong Perera, David Kum, Thong Wong Q Science (General) R Medicine (General) Japanese encephalitis virus (JEV) causes central nervous system neuronal injury and inflammation. A clear understanding of neuronal responses to JEV infection remains elusive. Using the Affymetrix array to investigate the transcriptome of infected SK-N-MC cells, 1316 and 2737 dysregulated genes (≥ 2/−2 fold change, P < 0.05) were found at 48 hours post-infection (hpi) and 60 hpi, respectively. The genes were mainly involved in anti-microbial responses, cell signalling, cellular function and maintenance, and cell death and survival. Among the most highly upregulated genes (≥ 10 folds, P < 0.05) were chemokines CCL5, CXCL11, IL8 and CXCL10. The upregulation and expression of CXCL11 were confirmed by qRT-PCR and immunofluorescence. Pathogen recognition receptors retinoic acid-inducible gene-1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA5) were also upregulated. Our results strongly suggest that neuronal cells play a significant role in immunity against JEV. CXCL11, RIG-1 and MDA5 and other cytokines may be important in neuropathogenesis Elsevier (New York, Academic Press.) 2018-10-16 Article PeerReviewed text en http://ir.unimas.my/id/eprint/22847/1/Shu%20Pin%20Yu.pdf Shu, Pin Yu and Kien, Chai Ong and Perera, David and Kum, Thong Wong (2018) Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5. Virology, 527. pp. 107-115. ISSN 0042-6822 https://www.journals.elsevier.com/virology DOI:org/10.1016/j.virol.2018.10.015 |
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Q Science (General) R Medicine (General) Shu, Pin Yu Kien, Chai Ong Perera, David Kum, Thong Wong Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 |
description |
Japanese encephalitis virus (JEV) causes central nervous system neuronal injury and inflammation. A clear
understanding of neuronal responses to JEV infection remains elusive. Using the Affymetrix array to investigate the transcriptome of infected SK-N-MC cells, 1316 and 2737 dysregulated genes (≥ 2/−2 fold change,
P < 0.05) were found at 48 hours post-infection (hpi) and 60 hpi, respectively. The genes were mainly involved
in anti-microbial responses, cell signalling, cellular function and maintenance, and cell death and survival.
Among the most highly upregulated genes (≥ 10 folds, P < 0.05) were chemokines CCL5, CXCL11, IL8 and
CXCL10. The upregulation and expression of CXCL11 were confirmed by qRT-PCR and immunofluorescence.
Pathogen recognition receptors retinoic acid-inducible gene-1 (RIG-1) and melanoma differentiation-associated
protein 5 (MDA5) were also upregulated. Our results strongly suggest that neuronal cells play a significant role in immunity against JEV. CXCL11, RIG-1 and MDA5 and other cytokines may be important in neuropathogenesis |
format |
Article |
author |
Shu, Pin Yu Kien, Chai Ong Perera, David Kum, Thong Wong |
author_facet |
Shu, Pin Yu Kien, Chai Ong Perera, David Kum, Thong Wong |
author_sort |
Shu, Pin Yu |
title |
Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 |
title_short |
Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 |
title_full |
Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 |
title_fullStr |
Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 |
title_full_unstemmed |
Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5 |
title_sort |
neuronal transcriptomic responses to japanese encephalitis virus infection with a special focus on chemokine cxcl11 and pattern recognition receptors rig-1 and mda5 |
publisher |
Elsevier (New York, Academic Press.) |
publishDate |
2018 |
url |
http://ir.unimas.my/id/eprint/22847/1/Shu%20Pin%20Yu.pdf http://ir.unimas.my/id/eprint/22847/ https://www.journals.elsevier.com/virology |
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1701166514398298112 |
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