Cryopreservation of raphanus sativus l. seeds using dehydration technique

• Main Author: Hazlina, bt Hamuddin.
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2003
• Subjects: QK Botany
• Online Access: http://ir.unimas.my/id/eprint/17928/3/Hazlina%20binti%20Hamuddin.pdf; http://ir.unimas.my/id/eprint/17928/

A study was conducted to evaluate the suitability of dehydration techniques to preserve Raphanus sativus L. seeds. Dehydration techniques used and compared were: silica gel, laminar flow and sucrose. Dehydration using silica gel for 24 hours gave the highest germination, which was 95.8%, and the moisture content was 3.05%. The highest germination percentage using laminar flow for 4 hours and sucrose at 0.2M for 120 minutes were 94.5% and 7.8% with the moisture content of 5% and 19.07% respectively. Regression analysis predicted that R. sativus could be stored for 3 months and 5 days if it is treated using silica gel. When seeds were treated using laminar flow, it can be stored in the liquid nitrogen for 2 months and 13 days. However, if the seeds were treated with sucrose, seeds can last for three days only.