Efficient and precise engineering of a 200 kb b-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system

• Main Authors: Narayanan, Kulathuramaiyer, Williamson, R., Zhang, Y., Stewart, AF, Ioannou, PA
• Format: Article
• Language: English
• Published: Stockton Press 1999
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/16746/1/Efficient.pdf; http://ir.unimas.my/id/eprint/16746/; http://www.nature.com/gt/journal/v6/n3/full/3300901a.html

Gene therapy studies require techniques that allow alter- adapted an inducible homologous recombination system ation of human genomic DNA sequences. Bacterial arti- for use in E. coli DH10B cells, the host strain for BACs ficial chromosome cloning systems (BACs/PACs) bridge and PACs. Using this sy...