Short homologies efficiently generate detectable homologous recombination events

• Main Authors: Osahor, Andrew N., Tan, Chau-Yan, Sim, Edmund U. H., Lee, Choon-Weng, Narayanan, Kulathuramaiyer
• Format: E-Article
• Language: English
• Published: Academic Press Inc. 2014
• Subjects: QD Chemistry
• Online Access: http://ir.unimas.my/id/eprint/17707/1/Short%20homologies%20efficiently%20generate%20detectable%20homologous%20%28abstract%29.pdf; http://ir.unimas.my/id/eprint/17707/; http://www.sciencedirect.com/science/article/pii/S0003269714002498

When recombineering bacterial artificial chromosomes (BACs), it is common practice to design the ends of the donor molecule with 50 bp of homology specifying its insertion site. We demonstrate that desired recombinants can be produced using intermolecular homologies as short as 15 bp. Although the...