Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1....
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Faculty of Food Science and Technology
2009
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Online Access: | http://ir.unimas.my/id/eprint/10108/1/zulkifli.pdf http://ir.unimas.my/id/eprint/10108/ https://www.researchgate.net/publication/288372852_Random_amplified_polymorphic_DNA-PCR_and_ERIC_PCR_analysis_on_Vibrio_parahaemolyticus_isolated_from_cockles_in_Padang_Indonesia |
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my.unimas.ir.101082023-01-26T04:55:17Z http://ir.unimas.my/id/eprint/10108/ Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, M. Q Science (General) QR Microbiology In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The Faculty of Food Science and Technology 2009 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10108/1/zulkifli.pdf Zulkifli, Y. and Alitheen, N.B. and Son, Radu and Raha, A.R and Samuel, Lihan and Yeap, S.K and Nishibuchi, M. (2009) Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16. pp. 141-150. ISSN 1985-4668 (Print), 2231-7546 (Online) https://www.researchgate.net/publication/288372852_Random_amplified_polymorphic_DNA-PCR_and_ERIC_PCR_analysis_on_Vibrio_parahaemolyticus_isolated_from_cockles_in_Padang_Indonesia |
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Q Science (General) QR Microbiology Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, M. Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
description |
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus
isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8
with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb.
Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based
on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to
52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven
different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into
4 major clusters with similarity between 56 to 86%. The |
format |
Article |
author |
Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, M. |
author_facet |
Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, M. |
author_sort |
Zulkifli, Y. |
title |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_short |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_full |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_fullStr |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_full_unstemmed |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_sort |
random amplified polymorphic dna-pcr and eric pcr analysis on vibrio parahaemolyticus isolated from cockles in padang, indonesia |
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Faculty of Food Science and Technology |
publishDate |
2009 |
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http://ir.unimas.my/id/eprint/10108/1/zulkifli.pdf http://ir.unimas.my/id/eprint/10108/ https://www.researchgate.net/publication/288372852_Random_amplified_polymorphic_DNA-PCR_and_ERIC_PCR_analysis_on_Vibrio_parahaemolyticus_isolated_from_cockles_in_Padang_Indonesia |
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1756063472651599872 |
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