Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1....
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Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Faculty of Food Science and Technology
2009
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/10108/1/zulkifli.pdf http://ir.unimas.my/id/eprint/10108/ https://www.researchgate.net/publication/288372852_Random_amplified_polymorphic_DNA-PCR_and_ERIC_PCR_analysis_on_Vibrio_parahaemolyticus_isolated_from_cockles_in_Padang_Indonesia |
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Summary: | In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus
isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8
with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb.
Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based
on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to
52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven
different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into
4 major clusters with similarity between 56 to 86%. The |
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