Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis
Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance tow...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IFRJ Publisher
2011
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10098/1/Samuel.pdf http://ir.unimas.my/id/eprint/10098/ |
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| Summary: | Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their
surrounding environment were determined. The levels of resistance of the E. coli isolates towards six
different antibiotics tested differed considerably. Though the isolates displayed resistance towards some of the
antibiotics tested, none of the isolates showed resistant towards norfloxacin, sulphametoxazole/trimethoprim
and chloramphenicol. RAPD-PCR analysis using single primer and primers combination clustered the E. coli
isolates into 3 and 5 groups, respectively. The results of this study suggest that the E. coli isolates from the catfish
and their surrounding environment derived from a mixture of sensitive and resistant strains with diverse genetic
contents. The use of the RAPD analysis is sufficiently discriminatory for the typing of the E. coli isolates. |
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