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Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum

(alupphYl/um lanigenum, a species that has a great potential in medicinal application, belongs to the Guttiferae family. The present study involves the isolation of DNA from this species. Three types of leaves were taken from the C. /anig>erum tree, namely matured, young and shoot leaves. T...

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Bibliographic Details
Main Author: Sukri, Berawi
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2003
Subjects:
S Agriculture (General)
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/46617/1/Sukri%20ft.pdf
http://ir.unimas.my/id/eprint/46617/
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spelling my.unimas.ir-466172024-11-13T06:51:45Z http://ir.unimas.my/id/eprint/46617/ Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum Sukri, Berawi S Agriculture (General) SB Plant culture (alupphYl/um lanigenum, a species that has a great potential in medicinal application, belongs to the Guttiferae family. The present study involves the isolation of DNA from this species. Three types of leaves were taken from the C. /anig>erum tree, namely matured, young and shoot leaves. The DNA isolated still contains RNA. RNase treatment was used to eliminate this impurity. The DNA obtained was stored at 20"C and 80"C up to I month and was examined tinder UV spectrophotometer to determine its concentration. Both temperatures were suitable for storage of the DNA without degradation. Storage of the DNA at such temperature could facilitate the isolation of useful genes from the plant for biotechnological applications. Universiti Malaysia Sarawak, (UNIMAS) 2003 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/46617/1/Sukri%20ft.pdf Sukri, Berawi (2003) Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
SB Plant culture
spellingShingle S Agriculture (General)
SB Plant culture
Sukri, Berawi
Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
description (alupphYl/um lanigenum, a species that has a great potential in medicinal application, belongs to the Guttiferae family. The present study involves the isolation of DNA from this species. Three types of leaves were taken from the C. /anig>erum tree, namely matured, young and shoot leaves. The DNA isolated still contains RNA. RNase treatment was used to eliminate this impurity. The DNA obtained was stored at 20"C and 80"C up to I month and was examined tinder UV spectrophotometer to determine its concentration. Both temperatures were suitable for storage of the DNA without degradation. Storage of the DNA at such temperature could facilitate the isolation of useful genes from the plant for biotechnological applications.
format Final Year Project Report
author Sukri, Berawi
author_facet Sukri, Berawi
author_sort Sukri, Berawi
title Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
title_short Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
title_full Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
title_fullStr Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
title_full_unstemmed Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
title_sort isolation and archiving of total genomic dna from medicinal plant, calophyllum lanigerum
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2003
url http://ir.unimas.my/id/eprint/46617/1/Sukri%20ft.pdf
http://ir.unimas.my/id/eprint/46617/
_version_ 1817848736583254016
score 13.223943

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