Isolation and Archiving of Total Genomic DNA From Medicinal Plant, Calophyllum lanigerum
(alupphYl/um lanigenum, a species that has a great potential in medicinal application, belongs to the Guttiferae family. The present study involves the isolation of DNA from this species. Three types of leaves were taken from the C. /anig>erum tree, namely matured, young and shoot leaves. T...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2003
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/46617/1/Sukri%20ft.pdf http://ir.unimas.my/id/eprint/46617/ |
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| Summary: | (alupphYl/um lanigenum, a species that has a great potential in medicinal application,
belongs to the Guttiferae family. The present study involves the isolation of DNA
from this species. Three types of leaves were taken from the C. /anig>erum tree,
namely matured, young and shoot leaves. The DNA isolated still contains RNA.
RNase treatment was used to eliminate this impurity. The DNA obtained was stored
at 20"C and 80"C up to I month and was examined tinder UV spectrophotometer
to determine its concentration. Both temperatures were suitable for storage of the
DNA without degradation. Storage of the DNA at such temperature could facilitate
the isolation of useful genes from the plant for biotechnological applications. |
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