Hybridization of glass-tethered oligonucleotide probes to target strands preannealed with labeled auxiliary oligonucleotides

• Main Authors: Maldonado-Rodriguez, Rogelio, Espinosa-Lara, Mercedes, Calixto-Suarez, Armando, Beattie, Wanda G., Beattie, Kenneth L.
• Format: Article
• Language: English
• Published: Humana Press Inc. 2010
• Subjects: CFTR gene; DNA chips; DNA probes; Genosensors; Mutation detection; Oligonucleotide arrays; Sequencing by hybridization
• Online Access: http://dspace.unimap.edu.my/xmlui/handle/123456789/9818

In this article we introduce a strategy of preannealing labeled auxiliary oligonucleotides to single-stranded target DNA, prior to hybridization of the DNA target to oligonucleotide arrays (genosensors) formed on glass slides for the purpose of mutation analysis. Human genomic DNA samples from norma...