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Hybridization of glass-tethered oligonucleotide probes to target strands preannealed with labeled auxiliary oligonucleotides

In this article we introduce a strategy of preannealing labeled auxiliary oligonucleotides to single-stranded target DNA, prior to hybridization of the DNA target to oligonucleotide arrays (genosensors) formed on glass slides for the purpose of mutation analysis. Human genomic DNA samples from norma...

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Bibliographic Details
Main Authors: Maldonado-Rodriguez, Rogelio, Espinosa-Lara, Mercedes, Calixto-Suarez, Armando, Beattie, Wanda G., Beattie, Kenneth L.
Format: Article
Language:English
Published: Humana Press Inc. 2010
Subjects:
CFTR gene
DNA chips
DNA probes
Genosensors
Mutation detection
Oligonucleotide arrays
Sequencing by hybridization
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/9818
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http://dspace.unimap.edu.my/xmlui/handle/123456789/9818

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