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Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens

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Bibliographic Details
Main Author: Kelly Lai, Jie Jing
Other Authors: Farizul Hafiz, Kasim, Dr.
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2021
Subjects:
Lipase
Bacillus amyloliquefaciens
Solid State Fermentation (SSF)
Bacteria
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73865
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spelling my.unimap-738652022-01-27T08:04:40Z Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens Kelly Lai, Jie Jing Farizul Hafiz, Kasim, Dr. School of Bioprocess Engineering Lipase Bacillus amyloliquefaciens Solid State Fermentation (SSF) Bacteria Access is limited to UniMAP community. Lipase is the main biocatalyst within the various industrial enzymes with proven industrial applications. The aim of this work was to produce halo-thermotolerant lipases by solid-state fermentation (SSF) using agro-industrial residues by Bacillus amyloliquefaciens. Seven agro-industrial residues substrates (coconut oil cake, coconut fibre, banana peels, oil palm empty fruit bunch, palm kernel cake, rice husk and sugarcane bagasse) were selected for solid state production of lipase at 37°C and 150 rpm. Among the tested supporting matrixes, coconut fibre with agitation SSF was found to be yielded maximum lipase and hence it was selected for further study. The effect of carbon source concentration, incubation temperature, agitation speed, sodium chloride (NaCl) concentration and carbon source concentration were evaluated using one-factorat-a-time technique. The maximum amount of lipase production was 0.1553 U/ml when glucose as carbon source, incubation temperature of 40°C, agitation speed of 200 rpm, 9% (w/v) NaCl concentration and 6% (w/v) glucose concentration were utilized. The thermostability of lipase from B. amyloliquefaciens showed active and stable in the temperature range from 30 to 60°C and its maximum lipolytic activity was at 60°C. Thus, it was observed that lipase from B. amyloliquefaciens was influenced by different cultural and process parameters. This study proposed the enzyme to be thermostable lipase. 2021 2022-01-27T08:04:40Z 2022-01-27T08:04:40Z 2017-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73865 en Universiti Malaysia Perlis (UniMAP)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Lipase
Bacillus amyloliquefaciens
Solid State Fermentation (SSF)
Bacteria
spellingShingle Lipase
Bacillus amyloliquefaciens
Solid State Fermentation (SSF)
Bacteria
Kelly Lai, Jie Jing
Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens
description Access is limited to UniMAP community.
author2 Farizul Hafiz, Kasim, Dr.
author_facet Farizul Hafiz, Kasim, Dr.
Kelly Lai, Jie Jing
format Learning Object
author Kelly Lai, Jie Jing
author_sort Kelly Lai, Jie Jing
title Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens
title_short Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens
title_full Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens
title_fullStr Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens
title_full_unstemmed Utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant Bacillus amyloliquefaciens
title_sort utilization of agro-industrial residues for improved lipase production by a halo-thermotolerant bacillus amyloliquefaciens
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2021
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73865
_version_ 1729704653726154752
score 13.214268

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