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DNA hybridization detection using 5-nm polysilicon nanogap structure

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Main Authors: Thikra S., Dhahi, Md Eaqub, Ali, Dr., Uda, Hashim, Prof. Dr., Nor Azah, Yusof, Ihsan M., Ali, M. J., Ashoor, A., Al-Roumy, H.A, Hadi
Other Authors: sthikra@yahoo.com
Format: Article
Language:English
Published: Bentham Science Publishers. 2014
Subjects:
Capacitance
Conductivity
DNA detection discrimination
Nanogap biosensor
Permittivity
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/32971
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spelling my.unimap-329712014-03-21T07:20:41Z DNA hybridization detection using 5-nm polysilicon nanogap structure Thikra S., Dhahi Md Eaqub, Ali, Dr. Uda, Hashim, Prof. Dr. Nor Azah, Yusof Ihsan M., Ali M. J., Ashoor A., Al-Roumy H.A, Hadi sthikra@yahoo.com eaqubali@um.edu.my uda@unimap.edu.my Capacitance Conductivity DNA detection discrimination Nanogap biosensor Permittivity Link to publisher's homepage at http://www.benthamscience.com/ This report described a polysilicon nanogap biosensor for the detection of target DNA hybridization which is a key step in biodiagnostics, gene expression profiling, environmental monitoring and forensic investigation. The detection was performed with a low cost dielectric analyzer which measured the changes in capacitance, conductance and permittivity of the nanogap electrodes upon target DNA hybridization. A conventional lithography coupled with thermal oxidation-based size reduction technique was used to fabricate the polysilicon nanogap electrodes. A layer of self-assembled amine functionalities coupled with non-covalently adsorbed gold nanoparticles was added onto the nanogap surface to create a binding chemistry for the thiol-modified probe DNA and to enhance the detection signal. The hybridization detection discrimination among the complementary, noncomplementary and single mismatch targets was reflected through the differences in capacitance, conductance and permittivity profiles of the biosensor. The detection limit of the polysilicon nanogap biosensor was 5 nmol/L of target DNA. 2014-03-21T07:20:41Z 2014-03-21T07:20:41Z 2013 Article Current Nanoscience, vol. 9(2), 2013, pages 283-287 1573-4137 http://www.eurekaselect.com/109029/article http://dspace.unimap.edu.my:80/dspace/handle/123456789/32971 en Bentham Science Publishers.
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Capacitance
Conductivity
DNA detection discrimination
Nanogap biosensor
Permittivity
spellingShingle Capacitance
Conductivity
DNA detection discrimination
Nanogap biosensor
Permittivity
Thikra S., Dhahi
Md Eaqub, Ali, Dr.
Uda, Hashim, Prof. Dr.
Nor Azah, Yusof
Ihsan M., Ali
M. J., Ashoor
A., Al-Roumy
H.A, Hadi
DNA hybridization detection using 5-nm polysilicon nanogap structure
description Link to publisher's homepage at http://www.benthamscience.com/
author2 sthikra@yahoo.com
author_facet sthikra@yahoo.com
Thikra S., Dhahi
Md Eaqub, Ali, Dr.
Uda, Hashim, Prof. Dr.
Nor Azah, Yusof
Ihsan M., Ali
M. J., Ashoor
A., Al-Roumy
H.A, Hadi
format Article
author Thikra S., Dhahi
Md Eaqub, Ali, Dr.
Uda, Hashim, Prof. Dr.
Nor Azah, Yusof
Ihsan M., Ali
M. J., Ashoor
A., Al-Roumy
H.A, Hadi
author_sort Thikra S., Dhahi
title DNA hybridization detection using 5-nm polysilicon nanogap structure
title_short DNA hybridization detection using 5-nm polysilicon nanogap structure
title_full DNA hybridization detection using 5-nm polysilicon nanogap structure
title_fullStr DNA hybridization detection using 5-nm polysilicon nanogap structure
title_full_unstemmed DNA hybridization detection using 5-nm polysilicon nanogap structure
title_sort dna hybridization detection using 5-nm polysilicon nanogap structure
publisher Bentham Science Publishers.
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/32971
_version_ 1643797038493597696
score 13.222552

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