Reducing the search space and time complexity of needleman-wunsch algorithm (global alignment) and smith waterman algorithm (local alignment) for DNA sequence alignment

• Main Author: Fatimah Noni, Muhamad
• Format: Thesis
• Language: English
• Published: Universiti Malaysia Perlis (UniMAP) 2014
• Subjects: Sequence comparison; DNA sequence alignment; Needleman-Wunsch algorithm; Dynamic Programming algorithm
• Online Access: http://dspace.unimap.edu.my:80/dspace/handle/123456789/31259

The fundamental procedure of analyzing sequence content is sequence comparison. Sequence comparison can be defined as the problem of finding which parts of the sequences are similar and which parts are different, namely comparing two sequences to identify similarities and differences between them. A typical approach to solve this problem is to find a good and reasonable alignment between the two sequences. The main research in this project is to align the DNA sequences by using the Needleman-Wunsch algorithm for global alignment and Smith-Waterman algorithm for local alignment based on the Dynamic Programming algorithm. The Dynamic Programming Algorithm is guaranteed to find optimal alignment by exploring all possible alignments and choosing the best through the scoring and traceback techniques. The algorithms proposed and evaluated are to reduce the gaps in aligning sequences as well as the length of the sequences aligned without compromising the quality or correctness of results. In this project, a study on how to apply the computational power of Parallel Computing to speed up the lengthy process of comparing sequences without having to compromise on the optimal results. Parallelization is only applied to the Needleman-Wunsch algorithm. In order to verify the accuracy and consistency of measurements obtained in Needleman-Wunsch and Smith-Waterman algorithms the data is compared with Emboss (global) and Emboss (local) with 600 strands test data. Results show that the Needle and Smith programs are reduced gaps and mismatch, but do not affect the accuracy. Results on the parallelization of Needleman-Wunsch algorithm shows that the parallelization is only efficient for 3000 length of DNA sequences and above, but does not show any improvement for less than 500 lengths of DNA sequences although using multiple core platforms.