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Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets

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Main Authors: Md. Eaqub, Ali, Uda, Hashim, Prof. Dr., Shuhaimi, Mustafa, Assoc. Prof. Dr., Yaacob, Che Man, Prof. Datuk Dr., Md. Abdul, Latif, Dr., Khondaker, Nurul Islam, Md. Zuki, Abu Bakar, Prof. Dr., Md. Majibar, Rahman
Other Authors: eaqubali@gmail.com
Format: Article
Language:English
Published: Vup Food Research Institute 2013
Subjects:
Authentication
Fortified chicken nuggets
Halal
Kosher
Meat emulsion
Taqman
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/23933
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spelling my.unimap-239332013-03-05T08:22:06Z Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa, Assoc. Prof. Dr. Yaacob, Che Man, Prof. Datuk Dr. Md. Abdul, Latif, Dr. Khondaker, Nurul Islam Md. Zuki, Abu Bakar, Prof. Dr. Md. Majibar, Rahman eaqubali@gmail.com uda@unimap.edu.my shuhaimi@biotech.upm.edu.my zuki@vet.upm.edu.my Authentication Fortified chicken nuggets Halal Kosher Meat emulsion Taqman Link to publisher's homepage at http://www.vup.sk/en/ A TaqMan real-time polymerase chain reaction was developed for the determination of pork adulteration in nuggets. The assay combined species-specific primers and TaqMan probe to target a 109 bp fragment of swine cytochrome b gene. Specificity test with 10 ng DNA of 11 different meat species yielded a quantification cycle (Cq) between 16.9 and 17.1 for pork, and negative results for other meats. Model experiment using chicken nuggets spiked with pork showed that the assay can quantify 100-0.01% of pork adulteration with a linear correlation (R 2 of 0.998, PCR efficiency of 91.1%, and relative error ≤ 5%). A plot of actual value against real-time PCR-predicted value yielded R2 of 0.999, and a very small (0.242) root mean square error of calibration. A strong correlation was found between the partial least square-predicted values and the values determined by real-time PCR. Random analysis of nuggets from pork, beef, chicken, mutton and chevon yielded Cq values between 18.2 and 18.6 for pork nuggets, and negative results for other meat nuggets. Finally, analysis of 27 commercial nuggets from each of the five common meat species revealed the presence of pork in 100%, 3.7%, 7.4%, 3.7% and 0% nuggets of pork, beef, mutton, chevon and chicken, respectively. 2013-03-05T08:22:06Z 2013-03-05T08:22:06Z 2012 Article Journal of Food and Nutrition Research, vol. 51 (1), 2012, pages 1-12 1336-8672 http://www.vup.sk/en/index.php?start&language=en&mainID=2&navID=34&version=2&volume=51&article=1834 http://hdl.handle.net/123456789/23933 en Vup Food Research Institute
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Authentication
Fortified chicken nuggets
Halal
Kosher
Meat emulsion
Taqman
spellingShingle Authentication
Fortified chicken nuggets
Halal
Kosher
Meat emulsion
Taqman
Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa, Assoc. Prof. Dr.
Yaacob, Che Man, Prof. Datuk Dr.
Md. Abdul, Latif, Dr.
Khondaker, Nurul Islam
Md. Zuki, Abu Bakar, Prof. Dr.
Md. Majibar, Rahman
Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
description Link to publisher's homepage at http://www.vup.sk/en/
author2 eaqubali@gmail.com
author_facet eaqubali@gmail.com
Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa, Assoc. Prof. Dr.
Yaacob, Che Man, Prof. Datuk Dr.
Md. Abdul, Latif, Dr.
Khondaker, Nurul Islam
Md. Zuki, Abu Bakar, Prof. Dr.
Md. Majibar, Rahman
format Article
author Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa, Assoc. Prof. Dr.
Yaacob, Che Man, Prof. Datuk Dr.
Md. Abdul, Latif, Dr.
Khondaker, Nurul Islam
Md. Zuki, Abu Bakar, Prof. Dr.
Md. Majibar, Rahman
author_sort Md. Eaqub, Ali
title Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
title_short Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
title_full Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
title_fullStr Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
title_full_unstemmed Taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
title_sort taqman real-time polymerase chain reaction for the determination of pork adulteration in meat nuggets
publisher Vup Food Research Institute
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/23933
_version_ 1643794089648324608
score 13.214268

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