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Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis

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Main Authors: Abdul Latif, M., M. Y., Omar, Tan, Soon Guan, Prof. Dr., Siti Shapor, Siraj, Prof. Dr., Md Eaqub, Ali, Mohd Rafii, Yusop, Assoc. Prof. Dr.
Other Authors: alatif1965@yahoo.com
Format: Article
Language:English
Published: FUNPEC-RP - Fundação de Pesquisas Científicas de Ribeirão Preto 2013
Subjects:
Brown planthopper
Determination
DNA contamination
Food assimilation
RAPD-PCR analysis
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/23375
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spelling my.unimap-233752013-02-06T03:40:07Z Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis Abdul Latif, M. M. Y., Omar Tan, Soon Guan, Prof. Dr. Siti Shapor, Siraj, Prof. Dr. Md Eaqub, Ali Mohd Rafii, Yusop, Assoc. Prof. Dr. alatif1965@yahoo.com eaqubali@gmail.com Brown planthopper Determination DNA contamination Food assimilation RAPD-PCR analysis Link to publisher's homepage at www.funpecrp.com.br Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilaparvata lugens, one from rice and the other from Leersia hexandra (Poaceae), a wetland forage grass, and we also investigated whether host plant DNA contaminates that of herbivore insects in extractions of whole insects. Ingestion and assimilation of food were reduced significantly when individuals derived from one host plant were caged on the other species. The bands, OPA3 (1.25), OPD3 (1.10), OPD3 (0.80), OPD3 (0.60), pUC/M13F (0.35), pUC/M13F (0.20), BOXAIR (0.50), peh#3 (0.50), and peh#3 (0.17) were found in both rice-infesting populations of brown planthopper and its host plant (rice). Similarly, the bands, OPA4 (1.00), OPB10 (0.70), OPD3 (0.90), OPD3 (0.80), OPD3 (0.60), pUC/ M13F (0.35), pUC/M13F (0.20), and BOXAIR (0.50) were found in both Leersia-infesting populations of brown planthopper and the host plant. So, it is clear that the DNA bands amplified in the host plants were also found in the extracts from the insects feeding on them 2013-02-06T03:40:07Z 2013-02-06T03:40:07Z 2012-01 Article Genetics and Molecular Research, vol.11 (1), 2012, pages 30-41 1676-5680 http://www.geneticsmr.com//year2012/vol11-1/pdf/gmr1402.pdf http://hdl.handle.net/123456789/23375 en FUNPEC-RP - Fundação de Pesquisas Científicas de Ribeirão Preto
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Brown planthopper
Determination
DNA contamination
Food assimilation
RAPD-PCR analysis
spellingShingle Brown planthopper
Determination
DNA contamination
Food assimilation
RAPD-PCR analysis
Abdul Latif, M.
M. Y., Omar
Tan, Soon Guan, Prof. Dr.
Siti Shapor, Siraj, Prof. Dr.
Md Eaqub, Ali
Mohd Rafii, Yusop, Assoc. Prof. Dr.
Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
description Link to publisher's homepage at www.funpecrp.com.br
author2 alatif1965@yahoo.com
author_facet alatif1965@yahoo.com
Abdul Latif, M.
M. Y., Omar
Tan, Soon Guan, Prof. Dr.
Siti Shapor, Siraj, Prof. Dr.
Md Eaqub, Ali
Mohd Rafii, Yusop, Assoc. Prof. Dr.
format Article
author Abdul Latif, M.
M. Y., Omar
Tan, Soon Guan, Prof. Dr.
Siti Shapor, Siraj, Prof. Dr.
Md Eaqub, Ali
Mohd Rafii, Yusop, Assoc. Prof. Dr.
author_sort Abdul Latif, M.
title Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
title_short Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
title_full Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
title_fullStr Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
title_full_unstemmed Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
title_sort food assimilated by two sympatric populations of the brown planthopper nilaparvata lugens (delphacidae) feeding on different host plants contaminates insect dna detected by rapd-pcr analysis
publisher FUNPEC-RP - Fundação de Pesquisas Científicas de Ribeirão Preto
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/23375
_version_ 1643793976092786688
score 13.214268

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