Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis.

• Main Authors: Latif, M.A., Md Yusoh, Omar, Tan, Soon Guan, Siraj, Siti Shapor, Ali, M.E., Yusop, Mohd Rafii
• Format: Article
• Language: English; English
• Published: Ribeirão Preto foundation for Scientific Research (FUNPEC) 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/24748/1/Food%20assimilated%20by%20two%20sympatric%20populations%20of%20the%20brown%20planthopper%20Nilaparvata%20lugens.pdf; http://psasir.upm.edu.my/id/eprint/24748/; http://www.jdb.uzh.ch/

Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of bro...