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What Happens When the Wrong Equation is Fitted to Data?

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Main Authors: Noorzaid Muhamad, Simon Brown, Kevin C Pedley, David C Simcock, UniKL RCMP
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Published: Islamia University of Bahawalpur 2013
Subjects:
distribution
enzyme kinetics
simulation
substrate inhibition
Online Access:http://ijes.info/2/4/42542403.pdf
http://ir.unikl.edu.my/jspui/handle/123456789/2264
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spelling my.unikl.ir-22642013-05-23T07:40:32Z What Happens When the Wrong Equation is Fitted to Data? Noorzaid Muhamad Simon Brown Kevin C Pedley David C Simcock UniKL RCMP distribution enzyme kinetics simulation substrate inhibition Open access Journal A common variant of the Michaelis-Menten model of enzyme kinetics involves inhibition by excess substrate. This phenomenon is known as substrate inhibition and the mathematical description of it requires an inhibition constant (Ki) as well as the usual kinetic parameters (Km and Vmax). Fitting the 3-parameter substrate inhibition expression to data that might reasonably be described by the 2-parameter Michaelis-Menten model yields biased estimates of Km and Vmax. Numerical simulations demonstrate that the extent of the bias is related to the magnitude of the estimated Ki. The quality of the data is particularly important in determining the size of Ki and, therefore, in the bias of the other parameters. Consideration of the residuals, statistical justification of the inclusion of extra parameters and reporting of the estimated values should be matters of routine. The estimates of Km and Vmax obtained from a three-parameter substrate inhibition model can only be compared with the corresponding estimates from the two-parameter Michaelis-Menten model with caution. 2013-05-23T07:40:32Z 2013-05-23T07:40:32Z 2012-12 2(4),p 533-542, http://ijes.info/2/4/42542403.pdf http://ir.unikl.edu.my/jspui/handle/123456789/2264 International Journal of Emerging Sciences; Islamia University of Bahawalpur
institution Universiti Kuala Lumpur
building UniKL Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kuala Lumpur
content_source UniKL Institutional Repository
url_provider http://ir.unikl.edu.my/
topic distribution
enzyme kinetics
simulation
substrate inhibition
spellingShingle distribution
enzyme kinetics
simulation
substrate inhibition
Noorzaid Muhamad
Simon Brown
Kevin C Pedley
David C Simcock
UniKL RCMP
What Happens When the Wrong Equation is Fitted to Data?
description Open access Journal
format
author Noorzaid Muhamad
Simon Brown
Kevin C Pedley
David C Simcock
UniKL RCMP
author_facet Noorzaid Muhamad
Simon Brown
Kevin C Pedley
David C Simcock
UniKL RCMP
author_sort Noorzaid Muhamad
title What Happens When the Wrong Equation is Fitted to Data?
title_short What Happens When the Wrong Equation is Fitted to Data?
title_full What Happens When the Wrong Equation is Fitted to Data?
title_fullStr What Happens When the Wrong Equation is Fitted to Data?
title_full_unstemmed What Happens When the Wrong Equation is Fitted to Data?
title_sort what happens when the wrong equation is fitted to data?
publisher Islamia University of Bahawalpur
publishDate 2013
url http://ijes.info/2/4/42542403.pdf
http://ir.unikl.edu.my/jspui/handle/123456789/2264
_version_ 1644484645022072832
score 13.222552

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