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Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)

An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoc...

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Bibliographic Details
Main Author: Willy, Yee
Format: Thesis
Language:English
Published: Terengganu : Universiti Malaysia Terengganu 2012
Subjects:
QR 82 .R45 W5 2011
Willy, Yee
Tesis FST 2011
Agrobacterium
Online Access:http://hdl.handle.net/123456789/1967
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id my.umt.ir-1967
record_format eprints
spelling my.umt.ir-19672012-10-08T03:43:16Z Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.) Willy, Yee QR 82 .R45 W5 2011 Willy, Yee Tesis FST 2011 Agrobacterium An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection. 2012-10-08T03:43:16Z 2012-10-08T03:43:16Z 2011-02 Thesis http://hdl.handle.net/123456789/1967 en application/pdf application/pdf Terengganu : Universiti Malaysia Terengganu
institution Universiti Malaysia Terengganu
building Perpustakaan Sultanah Nur Zahirah
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Terengganu
content_source UMT-IR
url_provider http://umt-ir.umt.edu.my:8080/
language English
topic QR 82 .R45 W5 2011
Willy, Yee
Tesis FST 2011
Agrobacterium
spellingShingle QR 82 .R45 W5 2011
Willy, Yee
Tesis FST 2011
Agrobacterium
Willy, Yee
Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
description An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection.
format Thesis
author Willy, Yee
author_facet Willy, Yee
author_sort Willy, Yee
title Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_short Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_full Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_fullStr Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_full_unstemmed Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
title_sort development of agrobacterium-mediated transformation method for marine microalgae (chlorella sp. and nannochloropsis sp.)
publisher Terengganu : Universiti Malaysia Terengganu
publishDate 2012
url http://hdl.handle.net/123456789/1967
_version_ 1738395583049105408
score 13.214268

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