Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoc...
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| Format: | Thesis |
| Language: | English |
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Terengganu : Universiti Malaysia Terengganu
2012
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| Online Access: | http://hdl.handle.net/123456789/1967 |
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| Summary: | An important step in the development of an efficient Agrobacterium-medialed
transformation system is the establishment of optimal conditions for efficient TDNA
transfer. Parameters known to influence the efficacy of Agrobacterium
tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation
temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS
expression two days post-infection. |
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