Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture
Commercial acacia gum (AG) used in this study is a premium-grade freeflowing powder. It is a gummy exudate composed of arabinogalactan branched polysaccharide, a biopolymer of arabinose and galactose. Also known as food additive, acacia gum (E414), which is presently marketed as a functional dietary...
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Lausanne : Frontiers Research Foundation
2023
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Online Access: | https://eprints.ums.edu.my/id/eprint/38138/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38138/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38138/ https://doi.org/10.3389/fmicb.2023.1245042 |
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my.ums.eprints.381382024-02-05T07:28:20Z https://eprints.ums.edu.my/id/eprint/38138/ Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture Muhamad Hanif Raw Hui Yan Tan Shahrul Razid Sarbini HD9720-9975 Manufacturing industries QP1-345 General Including influence of the environment Commercial acacia gum (AG) used in this study is a premium-grade freeflowing powder. It is a gummy exudate composed of arabinogalactan branched polysaccharide, a biopolymer of arabinose and galactose. Also known as food additive, acacia gum (E414), which is presently marketed as a functional dietary fiber to improve overall human gut health. The health effects may be related to the luminal pH regulation from the short-chain fatty acids (SCFA) production. Studies suggested that amylolytic and butyrogenic pathways are the major factors determining the SCFA outcome of AG in the lower gut. However, the primary bacteria involved in the fermentation have not been studied. This study aimed to investigate the putative primary degraders of acacia gum in the gut ecosystem. Isolation and identification of gum-fermenting bacteria were performed through enrichment culture fermentation. The experiment was conducted in an anaerobic chamber for 144 h in three stages. The study was conducted in triplicate using an anaerobic chamber system. This culture system allows specific responses to support only bacteria that are responsible for gum fermentation among the gut microbiota. Five bacterial strains were isolated and found to be gum-fermenting bacteria. Based on the 16s RNA sequence, the isolates matched to butyrateproducing Escherichia fergusonii, ATCC 35469. Lausanne : Frontiers Research Foundation 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38138/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38138/2/FULL%20TEXT.pdf Muhamad Hanif Raw and Hui Yan Tan and Shahrul Razid Sarbini (2023) Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture. Frontiers in Microbiology. pp. 1-11. ISSN 1664-302X https://doi.org/10.3389/fmicb.2023.1245042 |
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HD9720-9975 Manufacturing industries QP1-345 General Including influence of the environment Muhamad Hanif Raw Hui Yan Tan Shahrul Razid Sarbini Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture |
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Commercial acacia gum (AG) used in this study is a premium-grade freeflowing powder. It is a gummy exudate composed of arabinogalactan branched polysaccharide, a biopolymer of arabinose and galactose. Also known as food additive, acacia gum (E414), which is presently marketed as a functional dietary fiber to improve overall human gut health. The health effects may be related to the luminal pH regulation from the short-chain fatty acids (SCFA) production. Studies suggested that amylolytic and butyrogenic pathways are the major factors determining the SCFA outcome of AG in the lower gut. However, the primary bacteria involved in the fermentation have not been studied. This study aimed to investigate the putative primary degraders of acacia gum in the gut ecosystem. Isolation and identification of gum-fermenting bacteria were performed through enrichment culture fermentation. The experiment was conducted in an anaerobic chamber for 144 h in three stages. The study was conducted in triplicate using an anaerobic chamber system. This culture system allows specific responses to support only bacteria that are responsible for gum fermentation among the gut microbiota. Five bacterial strains were isolated and found to be gum-fermenting bacteria. Based on the 16s RNA sequence, the isolates matched to butyrateproducing Escherichia fergusonii, ATCC 35469. |
format |
Article |
author |
Muhamad Hanif Raw Hui Yan Tan Shahrul Razid Sarbini |
author_facet |
Muhamad Hanif Raw Hui Yan Tan Shahrul Razid Sarbini |
author_sort |
Muhamad Hanif Raw |
title |
Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture |
title_short |
Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture |
title_full |
Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture |
title_fullStr |
Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture |
title_full_unstemmed |
Identification of Acacia Gum Fermenting Bacteria from Pooled Human Feces using Anaerobic Enrichment Culture |
title_sort |
identification of acacia gum fermenting bacteria from pooled human feces using anaerobic enrichment culture |
publisher |
Lausanne : Frontiers Research Foundation |
publishDate |
2023 |
url |
https://eprints.ums.edu.my/id/eprint/38138/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38138/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38138/ https://doi.org/10.3389/fmicb.2023.1245042 |
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1792152900251680768 |
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13.209306 |