Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus

• Main Authors: Riteng Zhang, Peixin Wang, Xin Ma, Yifan Wu, Chen Luo, Li Qiu, Basit Zeshan, Zengqi Yang, Yefei Zhou, Xinglong Wang
• Format: Article
• Language: English; English
• Published: MDPI 2021
• Subjects: SF600-1100 Veterinary medicine
• Online Access: https://eprints.ums.edu.my/id/eprint/34813/1/Abstract.pdf; https://eprints.ums.edu.my/id/eprint/34813/2/Full%20text.pdf; https://eprints.ums.edu.my/id/eprint/34813/; https://www.mdpi.com/1999-4915/13/12/2531; https://doi.org/10.3390/v13122531

The TRS-mediated discontinuous transcription process is a hallmark of Arteriviruses. Precise assessment of the intricate sub genomic RNA (sg mRNA) populations is required to understand the kinetics of viral transcription. It is difficult to reconstruct and comprehensively quantify splicing events us...