Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas
Objectives: To characterise the mRNA expression patterns of early and advanced stage colorectal adenocarcinomas of Malaysian patients. Design: Comparative expression analysis. Setting and participants: We performed a combination of annealing control primer (ACP)-based PCR and reverse transcr...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/20524/1/Pair%20wise%20comparison%20analysis%20of%20differential%20expression%20of%20mRNAs%20in%20early%20and%20advanced%20stage%20primary%20colorectal%20adenocarcinomas.pdf https://eprints.ums.edu.my/id/eprint/20524/ https://doi.org/10.1136/bmjopen-2014- 004930 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.ums.eprints.20524 |
---|---|
record_format |
eprints |
spelling |
my.ums.eprints.205242018-07-17T05:56:10Z https://eprints.ums.edu.my/id/eprint/20524/ Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas Tze, Pheng Lau April Camilla Roslani Lay, Hoong Lian Hwa, Chia Chai Lee, Ping Chin Ida Hilmi Khean, Lee Goh Kek, Heng Chua QP Physiology Objectives: To characterise the mRNA expression patterns of early and advanced stage colorectal adenocarcinomas of Malaysian patients. Design: Comparative expression analysis. Setting and participants: We performed a combination of annealing control primer (ACP)-based PCR and reverse transcription-quantitative real-time PCR for the identification of differentially expressed genes (DEGs) associated with early and advanced stage primary colorectal tumours. We recruited four paired samples from patients with colorectal cancer (CRC) of Dukes’ A and B for the preliminary differential expression study, and a total of 27 paired samples, ranging from CRC stages I to IV, for subsequent confirmatory test. The tumouric samples were obtained from the patients with CRC undergoing curative surgical resection without preoperative chemoradiotherapy. The recruited patients with CRC were newly diagnosed with CRC, and were not associated with any hereditary syndromes, previously diagnosed cancer or positive family history of CRC. The paired non-cancerous tissue specimens were excised from macroscopically normal colonic mucosa distally located from the colorectal tumours. Primary and secondary outcome measures: The differential mRNA expression patterns of early and advanced stage colorectal adenocarcinomas compared with macroscopically normal colonic mucosa were characterised by ACP-based PCR and reverse transcription-quantitative real-time PCR. Results: The RPL35, RPS23 and TIMP1 genes were found to be overexpressed in both early and advanced stage colorectal adenocarcinomas ( p<0.05). However, the ARPC2 gene was significantly underexpressed in early colorectal adenocarcinomas, while the advanced stage primary colorectal tumours exhibited an additional overexpression of the C6orf173 gene ( p<0.05). Conclusions: We characterised two distinctive gene expression patterns to aid in the stratification of primary colorectal neoplasms among Malaysian patients with CRC. Further work can be done to assess and compare the mRNA expression levels of these identified DEGs between each CRC stage group, stages I–IV. 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/20524/1/Pair%20wise%20comparison%20analysis%20of%20differential%20expression%20of%20mRNAs%20in%20early%20and%20advanced%20stage%20primary%20colorectal%20adenocarcinomas.pdf Tze, Pheng Lau and April Camilla Roslani and Lay, Hoong Lian and Hwa, Chia Chai and Lee, Ping Chin and Ida Hilmi and Khean, Lee Goh and Kek, Heng Chua (2014) Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas. BMJ Open (ISI IF 2.06). https://doi.org/10.1136/bmjopen-2014- 004930 |
institution |
Universiti Malaysia Sabah |
building |
UMS Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sabah |
content_source |
UMS Institutional Repository |
url_provider |
http://eprints.ums.edu.my/ |
language |
English |
topic |
QP Physiology |
spellingShingle |
QP Physiology Tze, Pheng Lau April Camilla Roslani Lay, Hoong Lian Hwa, Chia Chai Lee, Ping Chin Ida Hilmi Khean, Lee Goh Kek, Heng Chua Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas |
description |
Objectives: To characterise the mRNA expression
patterns of early and advanced stage colorectal
adenocarcinomas of Malaysian patients.
Design: Comparative expression analysis.
Setting and participants: We performed a
combination of annealing control primer (ACP)-based
PCR and reverse transcription-quantitative real-time
PCR for the identification of differentially expressed
genes (DEGs) associated with early and advanced
stage primary colorectal tumours. We recruited four
paired samples from patients with colorectal cancer
(CRC) of Dukes’ A and B for the preliminary differential
expression study, and a total of 27 paired samples,
ranging from CRC stages I to IV, for subsequent
confirmatory test. The tumouric samples were obtained
from the patients with CRC undergoing curative
surgical resection without preoperative
chemoradiotherapy. The recruited patients with CRC
were newly diagnosed with CRC, and were not
associated with any hereditary syndromes, previously
diagnosed cancer or positive family history of CRC.
The paired non-cancerous tissue specimens were
excised from macroscopically normal colonic mucosa
distally located from the colorectal tumours.
Primary and secondary outcome measures: The
differential mRNA expression patterns of early and
advanced stage colorectal adenocarcinomas compared
with macroscopically normal colonic mucosa were
characterised by ACP-based PCR and reverse
transcription-quantitative real-time PCR.
Results: The RPL35, RPS23 and TIMP1 genes were
found to be overexpressed in both early and advanced
stage colorectal adenocarcinomas ( p<0.05). However,
the ARPC2 gene was significantly underexpressed in
early colorectal adenocarcinomas, while the advanced
stage primary colorectal tumours exhibited an additional
overexpression of the C6orf173 gene ( p<0.05).
Conclusions: We characterised two distinctive gene
expression patterns to aid in the stratification of
primary colorectal neoplasms among Malaysian
patients with CRC. Further work can be done to assess
and compare the mRNA expression levels of these
identified DEGs between each CRC stage group,
stages I–IV. |
format |
Article |
author |
Tze, Pheng Lau April Camilla Roslani Lay, Hoong Lian Hwa, Chia Chai Lee, Ping Chin Ida Hilmi Khean, Lee Goh Kek, Heng Chua |
author_facet |
Tze, Pheng Lau April Camilla Roslani Lay, Hoong Lian Hwa, Chia Chai Lee, Ping Chin Ida Hilmi Khean, Lee Goh Kek, Heng Chua |
author_sort |
Tze, Pheng Lau |
title |
Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas |
title_short |
Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas |
title_full |
Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas |
title_fullStr |
Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas |
title_full_unstemmed |
Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas |
title_sort |
pair-wise comparison analysis of differential expression of mrnas in early and advanced stage primary colorectal adenocarcinomas |
publishDate |
2014 |
url |
https://eprints.ums.edu.my/id/eprint/20524/1/Pair%20wise%20comparison%20analysis%20of%20differential%20expression%20of%20mRNAs%20in%20early%20and%20advanced%20stage%20primary%20colorectal%20adenocarcinomas.pdf https://eprints.ums.edu.my/id/eprint/20524/ https://doi.org/10.1136/bmjopen-2014- 004930 |
_version_ |
1760229726598725632 |
score |
13.18916 |