Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas
Objectives: To characterise the mRNA expression patterns of early and advanced stage colorectal adenocarcinomas of Malaysian patients. Design: Comparative expression analysis. Setting and participants: We performed a combination of annealing control primer (ACP)-based PCR and reverse transcr...
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Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
2014
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Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/20524/1/Pair%20wise%20comparison%20analysis%20of%20differential%20expression%20of%20mRNAs%20in%20early%20and%20advanced%20stage%20primary%20colorectal%20adenocarcinomas.pdf https://eprints.ums.edu.my/id/eprint/20524/ https://doi.org/10.1136/bmjopen-2014- 004930 |
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Summary: | Objectives: To characterise the mRNA expression
patterns of early and advanced stage colorectal
adenocarcinomas of Malaysian patients.
Design: Comparative expression analysis.
Setting and participants: We performed a
combination of annealing control primer (ACP)-based
PCR and reverse transcription-quantitative real-time
PCR for the identification of differentially expressed
genes (DEGs) associated with early and advanced
stage primary colorectal tumours. We recruited four
paired samples from patients with colorectal cancer
(CRC) of Dukes’ A and B for the preliminary differential
expression study, and a total of 27 paired samples,
ranging from CRC stages I to IV, for subsequent
confirmatory test. The tumouric samples were obtained
from the patients with CRC undergoing curative
surgical resection without preoperative
chemoradiotherapy. The recruited patients with CRC
were newly diagnosed with CRC, and were not
associated with any hereditary syndromes, previously
diagnosed cancer or positive family history of CRC.
The paired non-cancerous tissue specimens were
excised from macroscopically normal colonic mucosa
distally located from the colorectal tumours.
Primary and secondary outcome measures: The
differential mRNA expression patterns of early and
advanced stage colorectal adenocarcinomas compared
with macroscopically normal colonic mucosa were
characterised by ACP-based PCR and reverse
transcription-quantitative real-time PCR.
Results: The RPL35, RPS23 and TIMP1 genes were
found to be overexpressed in both early and advanced
stage colorectal adenocarcinomas ( p<0.05). However,
the ARPC2 gene was significantly underexpressed in
early colorectal adenocarcinomas, while the advanced
stage primary colorectal tumours exhibited an additional
overexpression of the C6orf173 gene ( p<0.05).
Conclusions: We characterised two distinctive gene
expression patterns to aid in the stratification of
primary colorectal neoplasms among Malaysian
patients with CRC. Further work can be done to assess
and compare the mRNA expression levels of these
identified DEGs between each CRC stage group,
stages I–IV. |
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