Tetraplex PCR assay involving double gene-sites discriminates beef and buffalo in Malaysian meat curry and burger products

• Main Authors: M.A. Motalib Hossain, Md. Eaqub Ali, Sharifah Bee Abd Hamid, S.M. Azad Hossain, Asing, Nina Naquiah Ahmad Nizar, Mohammad Nasir Uddin, Lokman Ali, Md. Asaduzzaman, Md. Jahurul Haque Akanda e
• Format: Article
• Language: English
• Published: Elsevier 2017
• Online Access: https://eprints.ums.edu.my/id/eprint/20329/1/Tetraplex%20PCR%20assay%20involving%20double%20gene.pdf; https://eprints.ums.edu.my/id/eprint/20329/

Replacement of beef by buffalo and vice versa is frequent in global markets, but their authentication is challenging in processed foods due to the fragmentation of most biomarkers including DNA. The shortening of target sequences through use of two target sites might ameliorate assay reliability be...