Total polyphenol, flavonoids content and antioxidant activity toward seeds of moringa oleifera
The project was undertaken to determine the total polyphenol content (TPC), total flavonoid content (TFC), antioxidative property, and the functional groups that present in the extracts of dichloromethane and methanol of seeds of Moringa oleifera. The percentage yield obtained for both dicholorom...
Saved in:
| Main Author: | |
|---|---|
| Format: | Academic Exercise |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/19583/1/Total%20polyphenol%2C%20flavonoids%20content%20.pdf https://eprints.ums.edu.my/id/eprint/19583/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The project was undertaken to determine the total polyphenol content (TPC), total
flavonoid content (TFC), antioxidative property, and the functional groups that present in
the extracts of dichloromethane and methanol of seeds of Moringa oleifera. The
percentage yield obtained for both dicholoromethane and methanol after the extraction
are 33.62% and 29.75% respectively. After the observation was done through Folin-Ciocalteu
method, the TPC obtained for dichloromethane and methanol are 151.0160 ±
0.0011 mg GAE/g and 114.0214 ± 0.0008 mg GAE/g respectively. In the determination
of TFC of these extracts through the aluminium chloride (AlCI₃) calometric method, it
was discovered that the extracts of dichloromethane and methanol bring the value of
159.3215 ± 0.0009 mg QE/g and 137.0982 ± 0.0007 mg QE/g respectively. The
oxidative property of M. oleifera were studied by using the 1,1-diphenyl-2-picrylhydrazyl
(DPPH) assay, and the IC50 values obtained for dichloromethane and methanol extracts
are 0.0157 mg/mL and 0.0078 mg/mL respectively. In this project, the determination of
antioxidant properties of both extracts was found to have almost the same properties
and the same antioxidative strength as the butylated hydroxytoluene (BHT) with IC50 of
0.0045. The IC50 value of the dichloromethane is the highest followed by the IC50 of
methanol extract and BHT with the lowest value. The determination of the presence of
functional groups through FTIR spectrophotometer indicates IR peaks that show a very
strong existence of the O-H group in the methanol extract. There are also weak peaks
present with different frequencies of different functional groups in both extracts. M.
oleifera has its own benefits as a good antioxidant, defensive ability in the plant and also
proves its quality as medicinal plant property. |
|---|