Nanosensor based formaldehyde detection of ikan tenggiri / narrow-barred spanish mackerel (Scomberomorus commerson)

• Main Author: Lum, Wai Mun
• Format: Academic Exercise
• Language: English
• Published: 2015
• Subjects: SF Animal culture
• Online Access: https://eprints.ums.edu.my/id/eprint/18522/1/Nanosensor%20based%20formaldehyde.pdf; https://eprints.ums.edu.my/id/eprint/18522/

Many conventional methods available that are used for determination of formaldehyde in fish samples, are complicated, time-consuming and costly. In this study, we develop an electrochemical nanosensor based on the modification of a glassy carbon electrode with chitosan, calcium oxide nanoparticles, ionic liquid, formaldehyde dehydrogenase enzyme and cofactor NAD+ for detection of formaldehyde in Scomberomorus commerson fish sample. Analysis was done using the cyclic voltammetry under optimized condition (0.1 V/S scan rate, pH 7.0 and 5 seconds). Methylene blue that was used as a redox indicator, was found to have significantly higher cathodic peak (I = -1.08 x 10⁻⁶ A) than without methylene blue (I = -0.89 x 10⁻⁶ A). Reproducibility, repeatability, storage stability and recovery assay in the fish sample were studied. Reproducibility of the electrode is good with RSD from 1.73 to 2.58 % while repeatability of the electrode is high with RSD from 5.04 to 6.30 %. Storage in the freezer did not significantly reduce (F=1.596, p=0.172) the concentration of formaldehyde in fish. Recovery of formaldehyde from Scomberomorus commerson fish sample spiked with 8 ppm formaldehyde was 8.79 %. Data was analysed using one way ANOVA. The formaldehyde nanosensor developed is a very simple and fast assay for analysing formaldehyde levels in fish samples. The findings of this study allows further optimization of formaldehyde nanosensor and also to analyse samples obtained from fish samples in the wet market.