Effect of organic additives on growth and development of Clinacanthus Nutans in vitro

• Format: Academic Exercise
• Language: English
• Published: Faculty of Sustainable Agriculture 2016
• Subjects: S Agriculture (General)
• Online Access: https://eprints.ums.edu.my/id/eprint/18042/1/Effect%20of%20organic%20additives%20on%20growth%20and%20development.pdf; https://eprints.ums.edu.my/id/eprint/18042/

An experiment was conducted at the Faculty of Sustainable Agriculture in University Malaysia Sabah to investigate the effect of organic additives on growth and development of Clinacanthus nutans. The nodal segment of C nutans was cultured on different MS medium supplemented with different types of organic additives. The media used were MSO (control), MS + 10% (vfv) coconut water, MS + 10% (v/v) banana homogenate, MS + 10% (v/v) potato homogenate and MS + 10% (v/v) tomato juice. The experiment was designed in a completely randomized designed (CRD) and each of the treatment was replicated 8 times. Data was collected according to number of leaves, length of regenerated shoots and number of roots. After 26 days of culture, results showed that MS medium supplemented with 10% (v/v) of coconut water showed the highest number of leaves (8.13±0.30), and highest length of regenerated shoot (2.71±0.07cm). While, the number of roots produced was (0.7S±0.71). The addition of 10% (vfv) potato homogenate showed the highest number (0.88±0.64) of roots. Thus, the presence of coconut water as organic additive in culture medium increase effectiveness of regeneration of nodal segment of C nutans.