Effect of organic additives on growth and development of Clinacanthus Nutans in vitro
An experiment was conducted at the Faculty of Sustainable Agriculture in University Malaysia Sabah to investigate the effect of organic additives on growth and development of Clinacanthus nutans. The nodal segment of C nutans was cultured on different MS medium supplemented with different types o...
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| Format: | Academic Exercise |
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| Language: | English |
| Published: |
Faculty of Sustainable Agriculture
2016
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/18042/1/Effect%20of%20organic%20additives%20on%20growth%20and%20development.pdf https://eprints.ums.edu.my/id/eprint/18042/ |
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| Summary: | An experiment was conducted at the Faculty of Sustainable Agriculture in University
Malaysia Sabah to investigate the effect of organic additives on growth and development
of Clinacanthus nutans. The nodal segment of C nutans was cultured on different MS
medium supplemented with different types of organic additives. The media used were
MSO (control), MS + 10% (vfv) coconut water, MS + 10% (v/v) banana homogenate,
MS + 10% (v/v) potato homogenate and MS + 10% (v/v) tomato juice. The experiment
was designed in a completely randomized designed (CRD) and each of the treatment
was replicated 8 times. Data was collected according to number of leaves, length of
regenerated shoots and number of roots. After 26 days of culture, results showed that
MS medium supplemented with 10% (v/v) of coconut water showed the highest number
of leaves (8.13±0.30), and highest length of regenerated shoot (2.71±0.07cm). While,
the number of roots produced was (0.7S±0.71). The addition of 10% (vfv) potato
homogenate showed the highest number (0.88±0.64) of roots. Thus, the presence of
coconut water as organic additive in culture medium increase effectiveness of
regeneration of nodal segment of C nutans. |
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