Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode

• Main Authors: Shafiquzzaman Siddiquee, Nor Azah Yusof, Abu Bakar Salleh, Tan, Soon Guan, Fatimah Abu Bakar
• Format: Article
• Language: English; English
• Published: Springer Nature 2012
• Subjects: Q1-390 Science (General); QK474.8-495 Spermatophyta. Phanerogams
• Online Access: https://eprints.ums.edu.my/id/eprint/1752/1/ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/1752/2/FULLTEXT.pdf; https://eprints.ums.edu.my/id/eprint/1752/; http://dx.doi.org/10.1007/s10008-011-1322-y; https://doi.org/10.1007/s10008-011-1322-y

Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the [EMIM][Otf]/ZnO/CHIT/AuE for detection of DNA hybridization were studied. Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of 1.0 × 10-18 to 1.82 × 10-4 mol L-1, and with the detection limit of 1.0 × 10-19 mol L-1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification of Trichoderma harzianum.