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Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)

The purification of papain using a reversed phase expanded bed adsorption chromatography (RP-EBAC)using a FastlineTM 10 EBAC column packed with AmberliteTM XAD7HP has been carried out in this study. An efficient large scale direct recovery of papain was developed and optimized from unclarified papay...

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Main Authors: Chong, Fui Chin, He, Yu Hai, Syed Mohd Saufi, Tuan Chik
Format: Article
Language:English
Published: Elsevier 2014
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/8002/1/fkksa-2014-yuhai-%20Purification%20of%20Papain%20from%20Unclarified%20Papaya%20Juice1.pdf
http://umpir.ump.edu.my/id/eprint/8002/
https://doi.org/10.1016/j.jiec.2014.01.035
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spelling my.ump.umpir.80022018-04-11T03:37:52Z http://umpir.ump.edu.my/id/eprint/8002/ Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) Chong, Fui Chin He, Yu Hai Syed Mohd Saufi, Tuan Chik TP Chemical technology The purification of papain using a reversed phase expanded bed adsorption chromatography (RP-EBAC)using a FastlineTM 10 EBAC column packed with AmberliteTM XAD7HP has been carried out in this study. An efficient large scale direct recovery of papain was developed and optimized from unclarified papaya juice feedstock in batch adsorption system. Enhancement of papain purity was further investigated in EBAC by stepwise elution strategy. High papain purity of 74.98% and high purification factor of 7.04 were obtained. This study shows a great potential of using two step elution RP-EBAC system to purify papain from unclarified papaya juice. Elsevier 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8002/1/fkksa-2014-yuhai-%20Purification%20of%20Papain%20from%20Unclarified%20Papaya%20Juice1.pdf Chong, Fui Chin and He, Yu Hai and Syed Mohd Saufi, Tuan Chik (2014) Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC). Journal of Industrial and Engineering Chemistry, 20 (6). pp. 4293-4297. ISSN 1226-086X https://doi.org/10.1016/j.jiec.2014.01.035 doi: 10.1016/j.jiec.2014.01.035
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Chong, Fui Chin
He, Yu Hai
Syed Mohd Saufi, Tuan Chik
Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
description The purification of papain using a reversed phase expanded bed adsorption chromatography (RP-EBAC)using a FastlineTM 10 EBAC column packed with AmberliteTM XAD7HP has been carried out in this study. An efficient large scale direct recovery of papain was developed and optimized from unclarified papaya juice feedstock in batch adsorption system. Enhancement of papain purity was further investigated in EBAC by stepwise elution strategy. High papain purity of 74.98% and high purification factor of 7.04 were obtained. This study shows a great potential of using two step elution RP-EBAC system to purify papain from unclarified papaya juice.
format Article
author Chong, Fui Chin
He, Yu Hai
Syed Mohd Saufi, Tuan Chik
author_facet Chong, Fui Chin
He, Yu Hai
Syed Mohd Saufi, Tuan Chik
author_sort Chong, Fui Chin
title Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
title_short Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
title_full Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
title_fullStr Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
title_full_unstemmed Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
title_sort purification of papain from unclarified papaya juice using reversed phase expanded bed adsorption chromatography (rp-ebac)
publisher Elsevier
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/8002/1/fkksa-2014-yuhai-%20Purification%20of%20Papain%20from%20Unclarified%20Papaya%20Juice1.pdf
http://umpir.ump.edu.my/id/eprint/8002/
https://doi.org/10.1016/j.jiec.2014.01.035
_version_ 1643665770767450112
score 13.160551

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