Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis

• Main Authors: Nur Husnina Iffah, Bakar, Thong, Chuan Lee, Mohd Hairul, Ab. Rahim, Raha, Ahmad Raus, Dayang Fredalina, Basri, Noor Suhana, Adzahar
• Format: Article
• Language: English
• Published: Trans Tech Publications 2020
• Subjects: T Technology (General)
• Online Access: http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf; http://umpir.ump.edu.my/id/eprint/30520/; https://doi.org/10.4028/www.scientific.net/MSF.981.265; https://doi.org/10.4028/www.scientific.net/MSF.981.265

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.