Microwave-assisted extraction of Vernonia amygdalina leaf for optimal recovery of total phenolic content
The microwave-assisted extraction conditions for the optimal yield of extracts and total phenolic content (TPC) from Vernonia amygdalina leaf were optimized using a face-centered central composite design of response surface methodology. The independent parameters were viz: irradiation time (5–15 m...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier GmbH
2018
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/21928/1/Microwave-assisted%20extraction%20of%20Vernonia%20amygdalina%20leaf%20for%20optimal.pdf http://umpir.ump.edu.my/id/eprint/21928/ https://doi.org/10.1016/j.jarmap.2018.04.004 https://doi.org/10.1016/j.jarmap.2018.04.004 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The microwave-assisted extraction conditions for the optimal yield of extracts and total phenolic content (TPC)
from Vernonia amygdalina leaf were optimized using a face-centered central composite design of response surface
methodology. The independent parameters were viz: irradiation time (5–15 min), microwave power
(400–600 W), temperature (90–110 °C), and feed-to-solvent ratio (1:8–1:12 g/mL). The optimal conditions for
highest yield of extract (22.34% w/w) and TPC (102.24 mg GAE/g d.w.) were obtained at irradiation time of
8 min, microwave power of 416 W, temperature of 100 °C, and feed-to-solvent ratio of 1:8 g/mL. The quadratic
model significantly (p < 0.0001) fitted the experimental data with R2 values of 0.9664 and 0.9885 for the
responses (extraction yield and TPC), respectively. Furthermore, the efficiency of MAE was compared with
Soxhlet, it was observed that the yields, antioxidant activity and functional groups from Fourier transform
infrared (FTIR) analysis was higher than that of the Soxhlet extract. |
|---|