Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)

• Main Authors: Rohaida, Che Man, R. M., Illias, Siti Zubaidah, Sulaiman, Siti Kholijah, Abdul Mudalip, Shalyda, Md Shaarani, Zatul Iffah, Mohd Arshad
• Format: Article
• Language: English
• Published: Bangladesh University of Engineering and Technology 2017
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/20606/1/Effect%20of%20the%20cross%20linkers%E2%80%99%20concentration%20on%20the%20immobilization%20of%20recombinant%20Escherichia%20coli%20cells%20on%20hollow%20fiber%20membrane%20for%20excretion%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29.pdf; http://umpir.ump.edu.my/id/eprint/20606/; https://www.banglajol.info/index.php/CERB/article/view/33810

The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in...