Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)

• Main Authors: Wan Siti Atikah, Wan Omar, Ahmad Ziad, Sulaiman, Azilah, Ajit, Yusuf, Chisti, Mohd Hairul, Ab Rahim, Adam Leow, Thean Chor
• Format: Conference or Workshop Item
• Language: English
• Published: Universiti Malaysia Pahang 2016
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf; http://umpir.ump.edu.my/id/eprint/15042/; http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip

Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into th...