Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)

Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into th...

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Main Authors: Wan Siti Atikah, Wan Omar, Ahmad Ziad, Sulaiman, Azilah, Ajit, Yusuf, Chisti, Mohd Hairul, Ab Rahim, Adam Leow, Thean Chor
Format: Conference or Workshop Item
Language:English
Published: Universiti Malaysia Pahang 2016
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Online Access:http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf
http://umpir.ump.edu.my/id/eprint/15042/
http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip
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spelling my.ump.umpir.150422020-06-09T03:15:59Z http://umpir.ump.edu.my/id/eprint/15042/ Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor TP Chemical technology Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. Universiti Malaysia Pahang 2016 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf Wan Siti Atikah, Wan Omar and Ahmad Ziad, Sulaiman and Azilah, Ajit and Yusuf, Chisti and Mohd Hairul, Ab Rahim and Adam Leow, Thean Chor (2016) Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3). In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016), 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 71-76.. http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Wan Siti Atikah, Wan Omar
Ahmad Ziad, Sulaiman
Azilah, Ajit
Yusuf, Chisti
Mohd Hairul, Ab Rahim
Adam Leow, Thean Chor
Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
description Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation.
format Conference or Workshop Item
author Wan Siti Atikah, Wan Omar
Ahmad Ziad, Sulaiman
Azilah, Ajit
Yusuf, Chisti
Mohd Hairul, Ab Rahim
Adam Leow, Thean Chor
author_facet Wan Siti Atikah, Wan Omar
Ahmad Ziad, Sulaiman
Azilah, Ajit
Yusuf, Chisti
Mohd Hairul, Ab Rahim
Adam Leow, Thean Chor
author_sort Wan Siti Atikah, Wan Omar
title Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_short Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_full Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_fullStr Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_full_unstemmed Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_sort transformation of ptrchis and puc8.2-14 into escherichia coli bl21 (de3)
publisher Universiti Malaysia Pahang
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf
http://umpir.ump.edu.my/id/eprint/15042/
http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip
_version_ 1672610875398160384
score 13.211869