Identification of Caryophyllene in Essential Oil from Various Parts of Cinnamomum Iners (Lauraceae)

• Main Author: Nita Nabila Kamarudin
• Format: Undergraduate Final Project Report
• Published: 2015
• Online Access: http://discol.umk.edu.my/id/eprint/6213/

Essential oils of herb bear various healing properties such as antifungal, antiseptic and also antibacterial. Caryophyllene is a natural bicyclic sesquiterpene in many essential oils and having potential as anti-inflammatory properties. Hence, this study was conducted to extract, isolate and analyse the presence of caryophyllene in the oils of C. iners leave, bark and twig. The samples of C. iners were collected at Agro-park UMK Jeli Campus. It was then subjected to hydro-distillation process for 5 hours. The distillate was collected and further isolated using hexane and anhydrous sodium sulphate (Na2SO4). The oils were then analysed using High Performance Liquid Chromatography (HPLC). The mobile phase used was acetonitrile-water (1:9 v/v). The hydro-distillation yielded of C. iners hydrosols which were extracted from various part of plant (leave, bark and twig). Secondary oils were obtained from hydrosol of leave, bark and twig extracted using hexane as organic solvent. The physical appearances of extracted oil are colourless with sticky and greasy while hydrosols are colourless. Both have pungent smell. Standard caryophyllene that was injected into HPLC appeared highest peak at retention time of 3.456 min. The highest peak of retention time of hydrosol C. iners leave, bark and twig were 3.661 min, 3.637 min and 6.623 min, respectively. As for the secondary 'oils, highest peak of retention time of C. iners leave, bark and twig were 3.663 min, 3.662 min and 3.668 min, respectively. For the secondary oils which are dissolved in hexane, the highest peak of retention time were 3.529 min, 3.534 min and 3.404 min corresponding to the leave, bark and twig, respectively. As a conclusion, two types of essential oils from various parts (leave, bark and twig) of C. iners were extracted. Furthermore, two major peaks were identified from the chromatogram of the HPLC analysis. There were no caryophyllene detected in hydrosol of C. iners twig and also secondary oil of C. iners bark.