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In vitro flowering of ethnomedicinal plant: borreria laevicaulis

The objective of the study was to develop the in vitro protocol for Borreria laevicaulis by applied the in vitro culture of mature nodal explants. The cultures were supplemented of MS media with various concentrations and combination of auxins and cytokinins (BAP, Kn, NAA and 2,4-D). After 8 weeks c...

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Main Author: Nik Nor Shahida Nik Pa
Format: Undergraduate Final Project Report
Published: 2013
Online Access:http://discol.umk.edu.my/id/eprint/5662/
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spelling my.umk.eprints.56622022-05-23T08:42:33Z http://discol.umk.edu.my/id/eprint/5662/ In vitro flowering of ethnomedicinal plant: borreria laevicaulis Nik Nor Shahida Nik Pa The objective of the study was to develop the in vitro protocol for Borreria laevicaulis by applied the in vitro culture of mature nodal explants. The cultures were supplemented of MS media with various concentrations and combination of auxins and cytokinins (BAP, Kn, NAA and 2,4-D). After 8 weeks cultured, the explants were observed and the maximum number of shoots, flowers and roots were formed in the MS medium that fortified with 2.0 mg/L BAP + 0.1 mg/L 2,4-D with the mean of shoot was 56.25, mean of flowers formed was 4.75 meanwhile mean for root induction was 16.50. From the research, the cytokinin like BAP was studied had a strong effect for the flower induction, meanwhile 2,4-D has greater result compare to NAA. Therefore, the in vitro flowering of Borreria laevicaulis was developed and this will lead for further study of flowering phenomenon n Rubiaceae family. 2013 Undergraduate Final Project Report NonPeerReviewed Nik Nor Shahida Nik Pa (2013) In vitro flowering of ethnomedicinal plant: borreria laevicaulis. Undergraduate Final Project Report thesis, Faculty Of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description The objective of the study was to develop the in vitro protocol for Borreria laevicaulis by applied the in vitro culture of mature nodal explants. The cultures were supplemented of MS media with various concentrations and combination of auxins and cytokinins (BAP, Kn, NAA and 2,4-D). After 8 weeks cultured, the explants were observed and the maximum number of shoots, flowers and roots were formed in the MS medium that fortified with 2.0 mg/L BAP + 0.1 mg/L 2,4-D with the mean of shoot was 56.25, mean of flowers formed was 4.75 meanwhile mean for root induction was 16.50. From the research, the cytokinin like BAP was studied had a strong effect for the flower induction, meanwhile 2,4-D has greater result compare to NAA. Therefore, the in vitro flowering of Borreria laevicaulis was developed and this will lead for further study of flowering phenomenon n Rubiaceae family.
format Undergraduate Final Project Report
author Nik Nor Shahida Nik Pa
spellingShingle Nik Nor Shahida Nik Pa
In vitro flowering of ethnomedicinal plant: borreria laevicaulis
author_facet Nik Nor Shahida Nik Pa
author_sort Nik Nor Shahida Nik Pa
title In vitro flowering of ethnomedicinal plant: borreria laevicaulis
title_short In vitro flowering of ethnomedicinal plant: borreria laevicaulis
title_full In vitro flowering of ethnomedicinal plant: borreria laevicaulis
title_fullStr In vitro flowering of ethnomedicinal plant: borreria laevicaulis
title_full_unstemmed In vitro flowering of ethnomedicinal plant: borreria laevicaulis
title_sort in vitro flowering of ethnomedicinal plant: borreria laevicaulis
publishDate 2013
url http://discol.umk.edu.my/id/eprint/5662/
_version_ 1763303585069662208
score 13.160551

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