Establishment of In Vitro of VitAto with Treatment of KIN and GA3 Hormones

• Main Author: Anisah Fasiha Mustaffa
• Format: Undergraduate Final Project Report
• Language: English
• Published: 2020
• Online Access: http://discol.umk.edu.my/id/eprint/4115/1/ANISAH%20FASIHA%20MUSTAFFA.pdf; http://discol.umk.edu.my/id/eprint/4115/

Micropropagation is the technique that involve the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature and a defined nutrient medium. Through micropropagation,not only huge amount of plants can be produced in short time, the specified or desired features can be added until it reaches the desired result of the plants. In this study, sweet potato(Ipomoea Batatas) is used.Sweet potato (Ipomoea Batatas) is one of the biggest consumed food in the world along with rice, maize, barley, wheat, cassava and potato.VitAto is another one of the new variety of sweet potato available that is known to have good potential in nutritional and functional values. At Malaysian Agriculture Research and Development Institute (MARDI), the researches have shown that this variety has higher content of Vitamin A (β-carotene). The problem statement of this study is a large number of loss will happen if we use conventional methods to propagate VitAto. For this study, the explant nodes will be used to be micropropagated. It is easier to be manage and surface sterilize the nodes. The explant node will be surface-sterilized before used. In this study, 10 different surface sterilizing method were used. Method D seem to be the best method for the explant survival with 37.33% rate of survival. This method use the combination of 70% ethanol, 5% hydrogen peroxide, 0.5% fungicide (Mancozeb) and 2 drops of Tween 20. The hormones used will be mixed into the media during media preparation. This study is aimed to increase the production of VitAto using micropropagation technique in optimum environment with the best ratio of hormones as supplement for the optimum growth of plant tissue. For this study, kinetin (KIN) and gibberellins (GA3) were used and the ratio of 0.01mg/l of KIN and 0.1mg/l GA3 is the best combination ratio for the root and shoot growth.