Cytotoxicity Effect Of Azadirachta Indica Extract On Vero Cells

• Main Author: Chin Xin Yee
• Format: Undergraduate Final Project Report
• Language: English; English
• Published: 2024
• Online Access: http://discol.umk.edu.my/id/eprint/14872/1/CHIN%20XIN%20YEE%20D20B00055.pdf; http://discol.umk.edu.my/id/eprint/14872/2/CHIN%20XIN%20YEE%20D20B00055.pdf; http://discol.umk.edu.my/id/eprint/14872/

Neem, or scientifically known as Azadirachta indica, is a fast growing evergreen plant that can be found in hot temperature countries like Malaysia, India and Pakistan, has its significant value as folk medicine. Previous research found that A. indica components make it a very good candidate for antifungal, anti-parasitism, antibacterial and anticancer. This study was conducted to determine the cytotoxic effects of this plant against normal mammalian cells such as Vero cells which are kidney cells derived from African Green Monkey. The study is aimed to have a better understanding on the safety profile of this plant against healthy mammalian cells. Cell culture was conducted on Vero cells, whereby the cells were treated with A. indica hexane extract at concentration ranging from 3.125 μg/mL to 100 μg/mL. The cultured cells incubated for a total of 72 hours, and accessed with MTS assay to assess the cell viability. The results of this study revealed that A. indica hexane extract showed no cytotoxic towards Vero cells, with MTS value of 11.13% at the concentration of 100 μg/mL against the normal mammalian cells, Vero cells,. This indicates that A. indica hexane extract is not cytotoxic towards normal healthy mammalian cells, which will not cause adverse effects to the normal functioning cells of the body, albeit possess a great anticancer property. Keywords: Neem, Azadirachta indica, hexane, evergreen, cytotoxic effects