A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram

Composite scaffolds of polycaprolactone (PCL) and bovine derived hydroxyapatite (HA) were produced by blending HA into PCL solution followed by electrospinning. This study mainly focused on studying the materials morphology, characteristics and interactions between the materials and cells. Six gr...

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Main Author: Haris, Akram
Format: Thesis
Published: 2014
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Online Access:http://studentsrepo.um.edu.my/7770/1/Thesis_Final%2DHaris_Bin_Akram.pdf
http://studentsrepo.um.edu.my/7770/
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spelling my.um.stud.77702017-10-25T09:01:30Z A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram Haris, Akram R Medicine (General) T Technology (General) Composite scaffolds of polycaprolactone (PCL) and bovine derived hydroxyapatite (HA) were produced by blending HA into PCL solution followed by electrospinning. This study mainly focused on studying the materials morphology, characteristics and interactions between the materials and cells. Six groups of scaffolds based on the weight ratio of HA in proportion to PCL were prepared (0%, 10%, 20%, 30%, 40% and 50%). The scaffolds were studied by infra-red spectroscopy (FTIR), 3D confocal laser microscopy and field emission scanning electron microscopy (FESEM) in order to determine the chemical and thermal properties as well as imaging the scaffold to cell interactions. Bone Marrow Stem Cell (BMSC) proliferation assays were carried out on Days 1, 7 and 14 using Resazurin, Deoxyribonucleic Acid (DNA) and Alkaline Phosphatase (ALP) measurements. DNA levels were used to identify cell presence and possible proliferation. ALP levels were used to indicate possible stem cell differentiation to osteoblasts. It was observed that with increasing amounts of HA in the PCL scaffold, the quantity and quality of cell attachment increased. Our study shows that the electrospun nanofibres of PCL incorporated with micro particles of HA could provide a scaffold that could be applied successfully in bone tissue engineering. 2014-09-29 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/7770/1/Thesis_Final%2DHaris_Bin_Akram.pdf Haris, Akram (2014) A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/7770/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic R Medicine (General)
T Technology (General)
spellingShingle R Medicine (General)
T Technology (General)
Haris, Akram
A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram
description Composite scaffolds of polycaprolactone (PCL) and bovine derived hydroxyapatite (HA) were produced by blending HA into PCL solution followed by electrospinning. This study mainly focused on studying the materials morphology, characteristics and interactions between the materials and cells. Six groups of scaffolds based on the weight ratio of HA in proportion to PCL were prepared (0%, 10%, 20%, 30%, 40% and 50%). The scaffolds were studied by infra-red spectroscopy (FTIR), 3D confocal laser microscopy and field emission scanning electron microscopy (FESEM) in order to determine the chemical and thermal properties as well as imaging the scaffold to cell interactions. Bone Marrow Stem Cell (BMSC) proliferation assays were carried out on Days 1, 7 and 14 using Resazurin, Deoxyribonucleic Acid (DNA) and Alkaline Phosphatase (ALP) measurements. DNA levels were used to identify cell presence and possible proliferation. ALP levels were used to indicate possible stem cell differentiation to osteoblasts. It was observed that with increasing amounts of HA in the PCL scaffold, the quantity and quality of cell attachment increased. Our study shows that the electrospun nanofibres of PCL incorporated with micro particles of HA could provide a scaffold that could be applied successfully in bone tissue engineering.
format Thesis
author Haris, Akram
author_facet Haris, Akram
author_sort Haris, Akram
title A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram
title_short A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram
title_full A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram
title_fullStr A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram
title_full_unstemmed A study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / Haris Akram
title_sort study on hydroxyapatite concentration in fibrous scaffolds and its effect on rat bone marrow stem cell proliferation / haris akram
publishDate 2014
url http://studentsrepo.um.edu.my/7770/1/Thesis_Final%2DHaris_Bin_Akram.pdf
http://studentsrepo.um.edu.my/7770/
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score 13.18916