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Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V...

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Main Authors: Hasima, N.N., Saidi, J., Thong, Kwai Lin, Ansary, A.
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2001
Subjects:
QR Microbiology
Online Access:http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf
http://eprints.um.edu.my/9969/
http://www.msmbb.my/index.php/archive-issues
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spelling my.um.eprints.99692021-10-22T01:41:56Z http://eprints.um.edu.my/9969/ Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA Hasima, N.N. Saidi, J. Thong, Kwai Lin Ansary, A. QR Microbiology Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia. Malaysian Society for Molecular Biology and Biotechnology 2001 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf Hasima, N.N. and Saidi, J. and Thong, Kwai Lin and Ansary, A. (2001) Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 147-150. ISSN 0128-7451 http://www.msmbb.my/index.php/archive-issues
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Hasima, N.N.
Saidi, J.
Thong, Kwai Lin
Ansary, A.
Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
description Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.
format Article
author Hasima, N.N.
Saidi, J.
Thong, Kwai Lin
Ansary, A.
author_facet Hasima, N.N.
Saidi, J.
Thong, Kwai Lin
Ansary, A.
author_sort Hasima, N.N.
title Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
title_short Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
title_full Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
title_fullStr Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
title_full_unstemmed Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
title_sort optimised conditions for pulsed field gel electrophoresis (pfge) separations of pasteurella multocida b:2 dna
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2001
url http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf
http://eprints.um.edu.my/9969/
http://www.msmbb.my/index.php/archive-issues
_version_ 1715190265760710656
score 13.160551

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